Antigenic peptides on the surface of Dirofilaria immitis microfilariae

Wesley K. Tamashiro, John P. Ehrenberg, David A. Levy, Alan Leroy Scott

Research output: Contribution to journalArticle

Abstract

Various methods of radioiodination were employed to identify peptides on the surface of Dirofilaria immitis microfilariae. Optimum surface radiolabelling occurred with the lactoperoxidase-catalyzed reaction. Two major peptides of 16 and 14 kDa were labelled by this method. These peptides were soluble in Nonidet P-40, were not glycosylated, and showed no signs of disulfide linkages. These peptides were immunoprecipitated by sera from D. immitis-infected dogs, but not by sera from uninfected dogs or sera from dogs with potentially cross-reactive nematode infections. Analysis of the 14 and 16 kDa peptides by two-dimensional gel electrophoresis revealed that the 16 kDa peptide was a single unit with a pI of 5.25 whereas the 14 kDa band was composed of three individual peptides with pI values ranging from 5.6 to 6.1. Iodination by chloramine T resulted in the same panel of labelled peptides but suffered from poor efficiency of 125I incorporation. The viability of microfilariae labelled by the standard Bolton-Hunter method decreased by 50% following the reaction which resulted in the labelling of a variety of internal components.

Original languageEnglish (US)
Pages (from-to)369-376
Number of pages8
JournalMolecular and Biochemical Parasitology
Volume18
Issue number3
DOIs
Publication statusPublished - 1986

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Keywords

  • Dirofilaria immitis
  • Microfilariae
  • Radiolabelling
  • Surface antigens

ASJC Scopus subject areas

  • Molecular Biology
  • Parasitology

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