Antigenic analysis of the repeat domain of the circumsporozoite protein of Plasmodium vivax

P. Romero, E. P. Heimer, S. Herrera, A. M. Felix, R. S. Nussenzweig, F. Zavala

Research output: Contribution to journalArticle

Abstract

In the present study we analyzed the fine specificity of mouse monoclonal and human polyclonal antibodies directed against the repeat domain of the circumsporozoite (CS) protein of the human malaria parasite, Plasmodium vivax. Five synthetic peptides, representing monomeric and dimeric repeats of this malarial antigen, were assayed for their capacity to inhibit the binding of these antibodies to a yeast-derived recombinant CS protein. The results revealed the existence of at least two distinct repeated overlapping epitopes in the CS protein of P. vivax. Furthermore, polyclonal sera contain antibodies which recognize additional determinants not represented by the synthetic repeat peptides. Some of these sera contain antibodies recognizing a region flanking the repeat domain (region I). The present findings are in contrast with the antibody response in rodents and humans to the Plasmodium falciparum CS protein, which is directed against a single repeated immunodominant epitope.

Original languageEnglish (US)
Pages (from-to)1679-1682
Number of pages4
JournalJournal of Immunology
Volume139
Issue number5
StatePublished - Dec 21 1987
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Fingerprint Dive into the research topics of 'Antigenic analysis of the repeat domain of the circumsporozoite protein of Plasmodium vivax'. Together they form a unique fingerprint.

  • Cite this

    Romero, P., Heimer, E. P., Herrera, S., Felix, A. M., Nussenzweig, R. S., & Zavala, F. (1987). Antigenic analysis of the repeat domain of the circumsporozoite protein of Plasmodium vivax. Journal of Immunology, 139(5), 1679-1682.