Antigenic analysis of the repeat domain of the circumsporozoite protein of Plasmodium vivax

P. Romero, E. P. Heimer, S. Herrera, A. M. Felix, R. S. Nussenzweig, Fidel P Zavala

Research output: Contribution to journalArticle

Abstract

In the present study we analyzed the fine specificity of mouse monoclonal and human polyclonal antibodies directed against the repeat domain of the circumsporozoite (CS) protein of the human malaria parasite, Plasmodium vivax. Five synthetic peptides, representing monomeric and dimeric repeats of this malarial antigen, were assayed for their capacity to inhibit the binding of these antibodies to a yeast-derived recombinant CS protein. The results revealed the existence of at least two distinct repeated overlapping epitopes in the CS protein of P. vivax. Furthermore, polyclonal sera contain antibodies which recognize additional determinants not represented by the synthetic repeat peptides. Some of these sera contain antibodies recognizing a region flanking the repeat domain (region I). The present findings are in contrast with the antibody response in rodents and humans to the Plasmodium falciparum CS protein, which is directed against a single repeated immunodominant epitope.

Original languageEnglish (US)
Pages (from-to)1679-1682
Number of pages4
JournalJournal of Immunology
Volume139
Issue number5
StatePublished - 1987
Externally publishedYes

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Plasmodium vivax
Antibodies
Vivax Malaria
Immunodominant Epitopes
Peptides
Plasmodium falciparum
Serum
Recombinant Proteins
Antibody Formation
Epitopes
Rodentia
Parasites
Yeasts
Antigens
Protein Domains
Proteins

ASJC Scopus subject areas

  • Immunology

Cite this

Romero, P., Heimer, E. P., Herrera, S., Felix, A. M., Nussenzweig, R. S., & Zavala, F. P. (1987). Antigenic analysis of the repeat domain of the circumsporozoite protein of Plasmodium vivax. Journal of Immunology, 139(5), 1679-1682.

Antigenic analysis of the repeat domain of the circumsporozoite protein of Plasmodium vivax. / Romero, P.; Heimer, E. P.; Herrera, S.; Felix, A. M.; Nussenzweig, R. S.; Zavala, Fidel P.

In: Journal of Immunology, Vol. 139, No. 5, 1987, p. 1679-1682.

Research output: Contribution to journalArticle

Romero, P, Heimer, EP, Herrera, S, Felix, AM, Nussenzweig, RS & Zavala, FP 1987, 'Antigenic analysis of the repeat domain of the circumsporozoite protein of Plasmodium vivax', Journal of Immunology, vol. 139, no. 5, pp. 1679-1682.
Romero P, Heimer EP, Herrera S, Felix AM, Nussenzweig RS, Zavala FP. Antigenic analysis of the repeat domain of the circumsporozoite protein of Plasmodium vivax. Journal of Immunology. 1987;139(5):1679-1682.
Romero, P. ; Heimer, E. P. ; Herrera, S. ; Felix, A. M. ; Nussenzweig, R. S. ; Zavala, Fidel P. / Antigenic analysis of the repeat domain of the circumsporozoite protein of Plasmodium vivax. In: Journal of Immunology. 1987 ; Vol. 139, No. 5. pp. 1679-1682.
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