Antigenic analysis of hematopoiesis. III. A hematopoietic progenitor cell surface antigen defined by a monoclonal antibody raised against KG-1a cells

C. I. Civin, L. C. Strauss, C. Brovall, M. J. Fackler, J. F. Schwartz, J. H. Shaper

Research output: Contribution to journalArticlepeer-review

Abstract

The anti-My-10 mouse monoclonal antibody was raised against the immature human myeloid cell line KG-1a and was selected for nonreactivity with mature human granulocytes. Anti-My-10 immunoprecipitated a KG-Ia cell surface protein with an apparent Mr of approximately 115 kD. We describe the binding of this antibody to human hematopoietic cell types and show that My-10 is expressed specifically on immature normal human marrow cells, including hematopoietic progenitor cells. My-10 is also expressed by leukemic marrow cells from a subpopulation of patients. Thus, this antibody allows the identification and purification of hematopoietic progenitor cells from normal human marrow and the subclassification of leukemia.

Original languageEnglish (US)
Pages (from-to)157-165
Number of pages9
JournalJournal of Immunology
Volume133
Issue number1
StatePublished - 1984
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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