Antigen-specific T lymphocyte clones. I. Characterization of a T lymphocyte clone expressing antigen-specific suppressive activity

M. Fresno, G. Nabel, L. McVay-Boudreau, H. Furthmayer, H. Cantor

Research output: Contribution to journalArticle

Abstract

We have generated continuously propagatable T lymphocyte clones to study antigen-specific T cell functions. All Ly-2+ clones mediate suppressive activity and secrete a characteristic pattern of polypeptides that differs from Ly-2-T cell clones. Cells of one clone, Cl.Ly23/4, specifically bind glycophorin from sheep erythrocytes (SRBC). After incubation with [35S]methionine, supernate material from this clone also contains biosynthetically labeled 70,000-mol wt proteins that specifically bind to SRBC and this binding is inhibited by glycophorin from sheep but not other erythrocytes. These antigen-binding 70,000-mol wt peptides specifically and completely suppress primary anti-SRBC responses generated by mixtures of primed Ly-1+2- cells and B cells. Suppression by these antigen-binding peptides reflects direct inhibition of T-helper activity.

Original languageEnglish (US)
Pages (from-to)1246-1259
Number of pages14
JournalJournal of Experimental Medicine
Volume153
Issue number5
DOIs
StatePublished - 1981
Externally publishedYes

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ASJC Scopus subject areas

  • Immunology

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