Antigen-specific IgA B memory cell responses to Shigella antigens elicited in volunteers immunized with live attenuated Shigella flexneri 2a oral vaccine candidates

J. K. Simon, M. Maciel, E. D. Weld, R. Wahid, M. F. Pasetti, W. L. Picking, K. L. Kotloff, M. M. Levine, M. B. Sztein

Research output: Contribution to journalArticle

Abstract

We studied the induction of antigen-specific IgA memory B cells (BM) in volunteers who received live attenuated Shigella flexneri 2a vaccines. Subjects ingested a single oral dose of 107, 108 or 109 CFU of S. flexneri 2a with deletions in guaBA (CVD 1204) or in guaBA, set and sen (CVD 1208). Antigen-specific serum and stool antibody responses to LPS and Ipa B were measured on days 0, 7, 14, 28 and 42. IgA BM cells specific to LPS, Ipa B and total IgA were assessed on days 0 and 28. We show the induction of significant LPS-specific IgA BM cells in anti-LPS IgA seroresponders. Positive correlations were found between anti-LPS IgA BM cells and anti-LPS IgA in serum and stool; IgA BM cell responses to IpaB were also observed. These BM cell responses are likely play an important role in modulating the magnitude and longevity of the humoral response.

Original languageEnglish (US)
Pages (from-to)185-192
Number of pages8
JournalClinical Immunology
Volume139
Issue number2
DOIs
StatePublished - May 1 2011

Keywords

  • B cell memory
  • IgA
  • Immunoglobulin A
  • Mucosal immunity
  • Shigella
  • Vaccine

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Fingerprint Dive into the research topics of 'Antigen-specific IgA B memory cell responses to Shigella antigens elicited in volunteers immunized with live attenuated Shigella flexneri 2a oral vaccine candidates'. Together they form a unique fingerprint.

  • Cite this