TY - JOUR
T1 - Antigen-presenting function of murine gonadal epithelial cell lines
AU - Housseau, Franck
AU - Rouas-Freiss, Nathalie
AU - Roy, Maguy
AU - Bidart, Jean Michel
AU - Guillet, Jean Gérard
AU - Bellet, Dominique
N1 - Funding Information:
1This work was supported by grants from the Association pour la Recherche sur le Cancer, Villejuif, and from the Laboratoire L. Lafon, Maisons-Alfort, France. 2To whom correspondence should be addressed.
PY - 1997/4/10
Y1 - 1997/4/10
N2 - Murine Leydig (TM3) and Sertoli (TM4) cell lines were studied as nonprofessional antigen-presenting cells using the antigen model of human choriogonadotropin (hCGα/β) and specific T-cell hybridomas. Both cell lines were treated with IFN-γ to induce I-A(d) and I-E(d) molecules expression. Only the TM3 cell line, which expressed MHC-class II molecules upon IFN-γ stimulation, was able to uptake, process, and present the human choriogonadotropin β subunit to related T-cell hybridomas. Interestingly, the TM3 cell line was incapable of presenting the human choriogonadotropin α subunit, the presentation of which, by classical APC, is highly efficient. Using T-cell hybridomas directed against the immunogenic regions of hCGα/β previously described in BALB/c mice, we showed that the TM3 cell line generated a narrower peptide repertoire than classical APC (i.e., B cells, macrophages, and dendritic cells). This experimental system suggests that Leydig cells could initiate, in vivo, an autoimmune process directed against gonadal tissues. In particular, such a mechanism has been evoked in experimental autoimmune orchitis.
AB - Murine Leydig (TM3) and Sertoli (TM4) cell lines were studied as nonprofessional antigen-presenting cells using the antigen model of human choriogonadotropin (hCGα/β) and specific T-cell hybridomas. Both cell lines were treated with IFN-γ to induce I-A(d) and I-E(d) molecules expression. Only the TM3 cell line, which expressed MHC-class II molecules upon IFN-γ stimulation, was able to uptake, process, and present the human choriogonadotropin β subunit to related T-cell hybridomas. Interestingly, the TM3 cell line was incapable of presenting the human choriogonadotropin α subunit, the presentation of which, by classical APC, is highly efficient. Using T-cell hybridomas directed against the immunogenic regions of hCGα/β previously described in BALB/c mice, we showed that the TM3 cell line generated a narrower peptide repertoire than classical APC (i.e., B cells, macrophages, and dendritic cells). This experimental system suggests that Leydig cells could initiate, in vivo, an autoimmune process directed against gonadal tissues. In particular, such a mechanism has been evoked in experimental autoimmune orchitis.
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U2 - 10.1006/cimm.1997.1092
DO - 10.1006/cimm.1997.1092
M3 - Article
C2 - 9140100
AN - SCOPUS:0031562669
SN - 0008-8749
VL - 177
SP - 93
EP - 101
JO - Cellular Immunology
JF - Cellular Immunology
IS - 1
ER -