Antigen-presenting function of murine gonadal epithelial cell lines

Franck Housseau, Nathalie Rouas-Freiss, Maguy Roy, Jean Michel Bidart, Jean Gérard Guillet, Dominique Bellet

Research output: Contribution to journalArticlepeer-review

11 Scopus citations


Murine Leydig (TM3) and Sertoli (TM4) cell lines were studied as nonprofessional antigen-presenting cells using the antigen model of human choriogonadotropin (hCGα/β) and specific T-cell hybridomas. Both cell lines were treated with IFN-γ to induce I-A(d) and I-E(d) molecules expression. Only the TM3 cell line, which expressed MHC-class II molecules upon IFN-γ stimulation, was able to uptake, process, and present the human choriogonadotropin β subunit to related T-cell hybridomas. Interestingly, the TM3 cell line was incapable of presenting the human choriogonadotropin α subunit, the presentation of which, by classical APC, is highly efficient. Using T-cell hybridomas directed against the immunogenic regions of hCGα/β previously described in BALB/c mice, we showed that the TM3 cell line generated a narrower peptide repertoire than classical APC (i.e., B cells, macrophages, and dendritic cells). This experimental system suggests that Leydig cells could initiate, in vivo, an autoimmune process directed against gonadal tissues. In particular, such a mechanism has been evoked in experimental autoimmune orchitis.

Original languageEnglish (US)
Pages (from-to)93-101
Number of pages9
JournalCellular Immunology
Issue number1
StatePublished - Apr 10 1997
Externally publishedYes

ASJC Scopus subject areas

  • Immunology


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