Antigen-induced sulfidopeptide leukotriene release from the guinea pig superfused trachea

Bradley J Undem, Walter C. Pickett, G. Kenneth Adams

Research output: Contribution to journalArticle

Abstract

The effect of antigen (ovalbumin) challenge on smooth muscle contraction and release of sulfidopeptide leukotrienes and histamine from superfused, actively sensitized guinea pig trachea was examined. Maximum concentrations of ovalbumin caused the release of 16 ± 4 ng/g immunoreactive sulfidopeptide leukotriene (i-LT) and 27 ± 3% of the endogenous histamine (x ± S.E.M., n = 19). High performance liquid chromatography combined with a sulfidopeptide leukotriene radioimmunoassay was used to demonstrate that on a molar basis, approximately 10% of the leukotriene immunoreactivity recovered was LTC4, 45% LTD4 and 45% LTE4. Indomethacin slightly increased ovalbumin-induced histamine release and substantially enhanced (3-fold) i-LT release from the trachea. Neither the profile nor rate of sulfidopeptide leukotriene release was altered by indomethacin. Indomethacin had no effect on the maximum amplitude of the antigen-induced contraction but significantly enhanced the magnitude of contraction observed after 10 min of antigen exposure. These results demonstrate that actively sensitized airways synthesize and release sulfidopeptide leukotrienes upon challenge with specific antigen and that endogenously formed LTC4 is efficiently metabolized to LTD4 and LTE4. The results with indomethacin support the hypothesis that indomethacin potentiates antigen-induced airway contraction in vitro by enhancing the release of mast cell associated mediators.

Original languageEnglish (US)
Pages (from-to)31-37
Number of pages7
JournalEuropean Journal of Pharmacology
Volume142
Issue number1
DOIs
StatePublished - Oct 6 1987
Externally publishedYes

Fingerprint

Leukotrienes
Trachea
Guinea Pigs
Indomethacin
Antigens
Ovalbumin
Leukotriene E4
Leukotriene D4
Leukotriene C4
Histamine
Histamine Release
Muscle Contraction
Mast Cells
Radioimmunoassay
Smooth Muscle
High Pressure Liquid Chromatography

Keywords

  • (Guinea-pig)
  • Histamine
  • Hypersensitivity (immediate)
  • Indomethacin
  • Leukotrienes
  • Trachea

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Pharmacology

Cite this

Antigen-induced sulfidopeptide leukotriene release from the guinea pig superfused trachea. / Undem, Bradley J; Pickett, Walter C.; Adams, G. Kenneth.

In: European Journal of Pharmacology, Vol. 142, No. 1, 06.10.1987, p. 31-37.

Research output: Contribution to journalArticle

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