Antigen expression of mouse spleen × thymocyte heterokaryons

David C. Allison, Paul Meier, Michele Majeune, Edward P. Cohen

Research output: Contribution to journalArticle

Abstract

The expression of Thy 1.2 and thymic leukemia (TL) antigens by heterokaryons of spleen cells of strain A mice (A-S) and AKR thymocytes (AKR-T) was determined. The A-S parental cells do not express TL antigens, although strain A thymocytes are TL-positive. Approximately 25% of A-S cells express Thy 1.2 antigens; however, AKR-T cells express a different Thy 1 antigen (Thy 1.1) and are phenotypically negative for TL expression. AKR-T × A-S heterokaryons were prepared with the aid of inactivated Sendai virus. Identification of heterokaryons was facilitated by prior isotopic labeling of AKR-T but not A-S cells, and the finding by autoradiography of binucleated cells with one radioactively labeled and one non-labeled nucleus. Antigenic expression of these fused cells was determined by exposure of the cells to specific antiserum and complement prior to autoradiography. 24 hr after fusion, fused cells were resistant to the cytotoxic effects of TL antiserum and fresh complement. However, a large proportion of these cells was lysed by treatment with antiserum directed against the Thy 1.2 antigen.

Original languageEnglish (US)
Pages (from-to)521-527
Number of pages7
JournalCell
Volume6
Issue number4
DOIs
StatePublished - Dec 1975

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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    Allison, D. C., Meier, P., Majeune, M., & Cohen, E. P. (1975). Antigen expression of mouse spleen × thymocyte heterokaryons. Cell, 6(4), 521-527. https://doi.org/10.1016/0092-8674(75)90041-0