Antifungal triazoles and polymorphonuclear leukocytes synergize to cause increased hyphal damage to Scedosporium prolificans and Scedosporium apiospermum

Cristina Gil-Lamaignere, Emmanuel Roilides, Juan Mosquera, Avgi Maloukou, Thomas J. Walsh

Research output: Contribution to journalArticle

Abstract

Scedosporium prolificans and Scedosporium apiospermum (Pseudallescheria boydii) cause pulmonary and disseminated infections refractory to most currently used antifungal agents in immunocompromised patients. We therefore investigated the potential antifungal activities of the triazoles itraconazole (ITC), voriconazole (VRC), and posaconazole (PSC) in combination with human polymorphonuclear leukocytes (PMNs) against the hyphae of these fungal pathogens. A colorimetric assay with (2,3-bis[2-methoxy-4-nitro-5-sulfophenyl]2H- tetrazolium-5-carboxanilide) sodium salt was used for the measurement of hyphal damage as an indicator of antifungal activity. We found that the newer triazoles VRC and PSC displayed synergistic effects with PMNs against S. prolificans hyphae after 24 h (P <0.05), whereas the effect of ITC in combination with PMNs was additive (P <0.01). All three triazoles displayed additive antifungal activities in combination with PMNs against S. apiospermum hyphae (P <0.05). The synergistic or additive effects that these triazoles exhibited, combined with the antifungal activities of human PMNs, may have important therapeutic implications for the management of infections due to S. prolificans and S. apiospermum.

Original languageEnglish (US)
Pages (from-to)2234-2237
Number of pages4
JournalAntimicrobial Agents and Chemotherapy
Volume46
Issue number7
DOIs
StatePublished - 2002
Externally publishedYes

Fingerprint

Scedosporium
Triazoles
Hyphae
Neutrophils
Itraconazole
Pseudallescheria
Antifungal Agents
Immunocompromised Host
Infection
Human Activities
Salts
Sodium
Lung

ASJC Scopus subject areas

  • Pharmacology (medical)

Cite this

Antifungal triazoles and polymorphonuclear leukocytes synergize to cause increased hyphal damage to Scedosporium prolificans and Scedosporium apiospermum. / Gil-Lamaignere, Cristina; Roilides, Emmanuel; Mosquera, Juan; Maloukou, Avgi; Walsh, Thomas J.

In: Antimicrobial Agents and Chemotherapy, Vol. 46, No. 7, 2002, p. 2234-2237.

Research output: Contribution to journalArticle

Gil-Lamaignere, Cristina ; Roilides, Emmanuel ; Mosquera, Juan ; Maloukou, Avgi ; Walsh, Thomas J. / Antifungal triazoles and polymorphonuclear leukocytes synergize to cause increased hyphal damage to Scedosporium prolificans and Scedosporium apiospermum. In: Antimicrobial Agents and Chemotherapy. 2002 ; Vol. 46, No. 7. pp. 2234-2237.
@article{a608e72cb3ad4778af3d9f6b60e9c85d,
title = "Antifungal triazoles and polymorphonuclear leukocytes synergize to cause increased hyphal damage to Scedosporium prolificans and Scedosporium apiospermum",
abstract = "Scedosporium prolificans and Scedosporium apiospermum (Pseudallescheria boydii) cause pulmonary and disseminated infections refractory to most currently used antifungal agents in immunocompromised patients. We therefore investigated the potential antifungal activities of the triazoles itraconazole (ITC), voriconazole (VRC), and posaconazole (PSC) in combination with human polymorphonuclear leukocytes (PMNs) against the hyphae of these fungal pathogens. A colorimetric assay with (2,3-bis[2-methoxy-4-nitro-5-sulfophenyl]2H- tetrazolium-5-carboxanilide) sodium salt was used for the measurement of hyphal damage as an indicator of antifungal activity. We found that the newer triazoles VRC and PSC displayed synergistic effects with PMNs against S. prolificans hyphae after 24 h (P <0.05), whereas the effect of ITC in combination with PMNs was additive (P <0.01). All three triazoles displayed additive antifungal activities in combination with PMNs against S. apiospermum hyphae (P <0.05). The synergistic or additive effects that these triazoles exhibited, combined with the antifungal activities of human PMNs, may have important therapeutic implications for the management of infections due to S. prolificans and S. apiospermum.",
author = "Cristina Gil-Lamaignere and Emmanuel Roilides and Juan Mosquera and Avgi Maloukou and Walsh, {Thomas J.}",
year = "2002",
doi = "10.1128/AAC.46.7.2234-2237.2002",
language = "English (US)",
volume = "46",
pages = "2234--2237",
journal = "Antimicrobial Agents and Chemotherapy",
issn = "0066-4804",
publisher = "American Society for Microbiology",
number = "7",

}

TY - JOUR

T1 - Antifungal triazoles and polymorphonuclear leukocytes synergize to cause increased hyphal damage to Scedosporium prolificans and Scedosporium apiospermum

AU - Gil-Lamaignere, Cristina

AU - Roilides, Emmanuel

AU - Mosquera, Juan

AU - Maloukou, Avgi

AU - Walsh, Thomas J.

PY - 2002

Y1 - 2002

N2 - Scedosporium prolificans and Scedosporium apiospermum (Pseudallescheria boydii) cause pulmonary and disseminated infections refractory to most currently used antifungal agents in immunocompromised patients. We therefore investigated the potential antifungal activities of the triazoles itraconazole (ITC), voriconazole (VRC), and posaconazole (PSC) in combination with human polymorphonuclear leukocytes (PMNs) against the hyphae of these fungal pathogens. A colorimetric assay with (2,3-bis[2-methoxy-4-nitro-5-sulfophenyl]2H- tetrazolium-5-carboxanilide) sodium salt was used for the measurement of hyphal damage as an indicator of antifungal activity. We found that the newer triazoles VRC and PSC displayed synergistic effects with PMNs against S. prolificans hyphae after 24 h (P <0.05), whereas the effect of ITC in combination with PMNs was additive (P <0.01). All three triazoles displayed additive antifungal activities in combination with PMNs against S. apiospermum hyphae (P <0.05). The synergistic or additive effects that these triazoles exhibited, combined with the antifungal activities of human PMNs, may have important therapeutic implications for the management of infections due to S. prolificans and S. apiospermum.

AB - Scedosporium prolificans and Scedosporium apiospermum (Pseudallescheria boydii) cause pulmonary and disseminated infections refractory to most currently used antifungal agents in immunocompromised patients. We therefore investigated the potential antifungal activities of the triazoles itraconazole (ITC), voriconazole (VRC), and posaconazole (PSC) in combination with human polymorphonuclear leukocytes (PMNs) against the hyphae of these fungal pathogens. A colorimetric assay with (2,3-bis[2-methoxy-4-nitro-5-sulfophenyl]2H- tetrazolium-5-carboxanilide) sodium salt was used for the measurement of hyphal damage as an indicator of antifungal activity. We found that the newer triazoles VRC and PSC displayed synergistic effects with PMNs against S. prolificans hyphae after 24 h (P <0.05), whereas the effect of ITC in combination with PMNs was additive (P <0.01). All three triazoles displayed additive antifungal activities in combination with PMNs against S. apiospermum hyphae (P <0.05). The synergistic or additive effects that these triazoles exhibited, combined with the antifungal activities of human PMNs, may have important therapeutic implications for the management of infections due to S. prolificans and S. apiospermum.

UR - http://www.scopus.com/inward/record.url?scp=0035984801&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035984801&partnerID=8YFLogxK

U2 - 10.1128/AAC.46.7.2234-2237.2002

DO - 10.1128/AAC.46.7.2234-2237.2002

M3 - Article

C2 - 12069979

AN - SCOPUS:0035984801

VL - 46

SP - 2234

EP - 2237

JO - Antimicrobial Agents and Chemotherapy

JF - Antimicrobial Agents and Chemotherapy

SN - 0066-4804

IS - 7

ER -