Antibody specificity profiling using protein microarrays

Pedro Ramos-López, José Irizarry, Ignacio Pino, Seth Blackshaw

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Antibodies are the most widely used reagent for isolation and detection of specific proteins. However, using antibodies that are not highly specific in these studies can generate inaccurate and misleading data. Protein microarrays offer a platform by which antibody cross-reactivity against a broad range of cellular antigens can be simultaneously and quantitatively profiled. This protocol describes in detail the process of array pretreatment, antibody binding, washing, scanning and quantitative analysis of antibody specificity.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages223-229
Number of pages7
DOIs
StatePublished - Jan 1 2018

Publication series

NameMethods in Molecular Biology
Volume1785
ISSN (Print)1064-3745

Keywords

  • Antibody specificity
  • Cross-reactivity
  • Monoclonal antibodies
  • Protein microarray

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Ramos-López, P., Irizarry, J., Pino, I., & Blackshaw, S. (2018). Antibody specificity profiling using protein microarrays. In Methods in Molecular Biology (pp. 223-229). (Methods in Molecular Biology; Vol. 1785). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7841-0_14