TY - JOUR
T1 - Antibody response to human papillomavirus (HPV) type 11 in children with juvenile-onset recurrent respiratory papillomatosis (RRP)
AU - Bonnez, William
AU - Kashimat, Haskins K.
AU - Leventhal, Brigid
AU - Mounts, Phoebe
AU - Rose, Robert C.
AU - Reichman, Richard C.
AU - Shah, Keerti V.
N1 - Funding Information:
We are grateful to John W. Kreider for the use of the HPV-1 lHarshey strain. We are also indebted to Came DaRin for excellent technical assistance. We thank Debbie Reiss and the staff of the xenograft facility at the University of Rochester for their support in the care of the animals. David S. Strike provided us with some of the HPV-1 l-infected human xenografts. Reid Mattison and Elizabeth Woodward graciously gave us access to human neonatal foreskins. This study has been partially supported by Contract NOl-AI-82509 from the National Institute of Allergy and Infectious Diseases (R. C. Reich-man), and Grant POl-Al-16959 from the U.S. Public Health Service (K. V. Shah).
PY - 1992/5
Y1 - 1992/5
N2 - We previously established, using an ELISA, the presence of specific antibodies directed at human papillomavirus (HPV) type 11 virions in the sera of patients with condylomata acuminata, mostly a disease of young adults that, like recurrent respiratory papillomatosis (RRP), is caused by two closely related HPVs, types 6 and 11. The present study was done to investigate if children with RRP can make viral-specific antibodies to an infection that is acquired at birth. Using the same ELISA, we studied the sera of 32 children with biopsy-documented juvenile-onset RRP and compared them to the sera of 31 control children. The median (and interquartile range) of the OD values in the controls and the cases was 0.078 (0.003, 0.101) and 0.230 (0.063, 0.725), respectively, a statistically significant difference (P = 0.001). Among the cases, there was no difference in seroreactivity between children with HPV-11-induced RRP and those with HPV-6-induced RRP (P = 0.31). Since HPV-11 viral particles do bind to the ELISA plate and remain intact and accessible to antibodies, we conclude that children with RRP, like adults with condylomata acuminata, develop antibodies directed at HPV-11 virions.
AB - We previously established, using an ELISA, the presence of specific antibodies directed at human papillomavirus (HPV) type 11 virions in the sera of patients with condylomata acuminata, mostly a disease of young adults that, like recurrent respiratory papillomatosis (RRP), is caused by two closely related HPVs, types 6 and 11. The present study was done to investigate if children with RRP can make viral-specific antibodies to an infection that is acquired at birth. Using the same ELISA, we studied the sera of 32 children with biopsy-documented juvenile-onset RRP and compared them to the sera of 31 control children. The median (and interquartile range) of the OD values in the controls and the cases was 0.078 (0.003, 0.101) and 0.230 (0.063, 0.725), respectively, a statistically significant difference (P = 0.001). Among the cases, there was no difference in seroreactivity between children with HPV-11-induced RRP and those with HPV-6-induced RRP (P = 0.31). Since HPV-11 viral particles do bind to the ELISA plate and remain intact and accessible to antibodies, we conclude that children with RRP, like adults with condylomata acuminata, develop antibodies directed at HPV-11 virions.
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U2 - 10.1016/0042-6822(92)90770-P
DO - 10.1016/0042-6822(92)90770-P
M3 - Article
C2 - 1314464
AN - SCOPUS:0026723863
VL - 188
SP - 384
EP - 387
JO - Virology
JF - Virology
SN - 0042-6822
IS - 1
ER -