Antibody response to common viruses and human leukocyte antigen-DRB1 in pediatric multiple sclerosis

Emmanuelle Waubant, Ellen Mahar Mowry, Lauren Krupp, Tanuja Chitnis, E. Ann Yeh, Nancy Kuntz, Jayne Ness, Anita Belman, Maria Milazzo, Mark Gorman, Bianca Weinstock-Guttman, Moses Rodriguez, Judith A. James

Research output: Contribution to journalArticle

Abstract

Background: As remote infections with common herpes viruses are associated with modulation of the risk of multiple sclerosis (MS), we hypothesized that antibody concentrations against these viruses may further modify risk. As many common viruses are first encountered during childhood, pediatric MS offer a unique opportunity to investigate more closely their influence on susceptibility. Our aim was to determine if MS patients who were positive for these viruses had higher levels of antibodies to these viruses. We also assessed whether human leukocyte antigen (HLA)-DRB1*1501 genotype influenced viral antibody levels. Methods: Antibody response levels toward Epstein Barr virus (EBV), cytomegalovirus (CMV), and herpes simplex virus (HSV)-1, and HLA-DRB1*1501 status were determined in pediatric MS patients (n=189) and controls (n=38). Multivariate analyses were used, adjusted for age, gender, race, ethnicity and use of disease-modifying therapies. Results: The antibody concentrations against EBV (Epstein-Barr nuclear antigen 1 (EBNA-1), viral capsid antigen (VCA) and early antigen (EA)), CMV and HSV-1 were similar between pediatric MS patients and controls positive for seroconversion against the virus of interest. EBNA-1 humoral responses were higher in HLA-DRB1 positive individuals (p=0.005) whereas other viral humoral responses were similar in HLA-DRB1 positive and negative individuals. Conclusion: Among those positive for EBNA-1, MS patients did not have higher levels of antibody response to EBNA- 1: however, titers for EBNA-1 were higher in those who were HLA-DRB1 positive. This suggests that genotype might influence the humoral response to EBV. Whether other genotypes influence antibody response to other viruses remains to be determined.

Original languageEnglish (US)
Pages (from-to)891-895
Number of pages5
JournalMultiple Sclerosis
Volume19
Issue number7
DOIs
StatePublished - 2013
Externally publishedYes

Fingerprint

HLA Antigens
Multiple Sclerosis
Antibody Formation
Nuclear Antigens
Pediatrics
Viruses
Human Herpesvirus 4
Genotype
Human Herpesvirus 1
Antibodies
Viral Antibodies
Viral Antigens
Capsid
Cytomegalovirus
Multivariate Analysis
Infection

Keywords

  • cytomegalovirus
  • DRB1
  • Epstein-Barr virus
  • gene-environment interaction
  • herpes simplex virus-1
  • Multiple sclerosis susceptibility
  • pediatric multiple sclerosis
  • risk factors

ASJC Scopus subject areas

  • Clinical Neurology
  • Neurology

Cite this

Antibody response to common viruses and human leukocyte antigen-DRB1 in pediatric multiple sclerosis. / Waubant, Emmanuelle; Mowry, Ellen Mahar; Krupp, Lauren; Chitnis, Tanuja; Yeh, E. Ann; Kuntz, Nancy; Ness, Jayne; Belman, Anita; Milazzo, Maria; Gorman, Mark; Weinstock-Guttman, Bianca; Rodriguez, Moses; James, Judith A.

In: Multiple Sclerosis, Vol. 19, No. 7, 2013, p. 891-895.

Research output: Contribution to journalArticle

Waubant, E, Mowry, EM, Krupp, L, Chitnis, T, Yeh, EA, Kuntz, N, Ness, J, Belman, A, Milazzo, M, Gorman, M, Weinstock-Guttman, B, Rodriguez, M & James, JA 2013, 'Antibody response to common viruses and human leukocyte antigen-DRB1 in pediatric multiple sclerosis', Multiple Sclerosis, vol. 19, no. 7, pp. 891-895. https://doi.org/10.1177/1352458512469693
Waubant, Emmanuelle ; Mowry, Ellen Mahar ; Krupp, Lauren ; Chitnis, Tanuja ; Yeh, E. Ann ; Kuntz, Nancy ; Ness, Jayne ; Belman, Anita ; Milazzo, Maria ; Gorman, Mark ; Weinstock-Guttman, Bianca ; Rodriguez, Moses ; James, Judith A. / Antibody response to common viruses and human leukocyte antigen-DRB1 in pediatric multiple sclerosis. In: Multiple Sclerosis. 2013 ; Vol. 19, No. 7. pp. 891-895.
@article{3681fea6b65848e0a25f261ffcc0fe19,
title = "Antibody response to common viruses and human leukocyte antigen-DRB1 in pediatric multiple sclerosis",
abstract = "Background: As remote infections with common herpes viruses are associated with modulation of the risk of multiple sclerosis (MS), we hypothesized that antibody concentrations against these viruses may further modify risk. As many common viruses are first encountered during childhood, pediatric MS offer a unique opportunity to investigate more closely their influence on susceptibility. Our aim was to determine if MS patients who were positive for these viruses had higher levels of antibodies to these viruses. We also assessed whether human leukocyte antigen (HLA)-DRB1*1501 genotype influenced viral antibody levels. Methods: Antibody response levels toward Epstein Barr virus (EBV), cytomegalovirus (CMV), and herpes simplex virus (HSV)-1, and HLA-DRB1*1501 status were determined in pediatric MS patients (n=189) and controls (n=38). Multivariate analyses were used, adjusted for age, gender, race, ethnicity and use of disease-modifying therapies. Results: The antibody concentrations against EBV (Epstein-Barr nuclear antigen 1 (EBNA-1), viral capsid antigen (VCA) and early antigen (EA)), CMV and HSV-1 were similar between pediatric MS patients and controls positive for seroconversion against the virus of interest. EBNA-1 humoral responses were higher in HLA-DRB1 positive individuals (p=0.005) whereas other viral humoral responses were similar in HLA-DRB1 positive and negative individuals. Conclusion: Among those positive for EBNA-1, MS patients did not have higher levels of antibody response to EBNA- 1: however, titers for EBNA-1 were higher in those who were HLA-DRB1 positive. This suggests that genotype might influence the humoral response to EBV. Whether other genotypes influence antibody response to other viruses remains to be determined.",
keywords = "cytomegalovirus, DRB1, Epstein-Barr virus, gene-environment interaction, herpes simplex virus-1, Multiple sclerosis susceptibility, pediatric multiple sclerosis, risk factors",
author = "Emmanuelle Waubant and Mowry, {Ellen Mahar} and Lauren Krupp and Tanuja Chitnis and Yeh, {E. Ann} and Nancy Kuntz and Jayne Ness and Anita Belman and Maria Milazzo and Mark Gorman and Bianca Weinstock-Guttman and Moses Rodriguez and James, {Judith A.}",
year = "2013",
doi = "10.1177/1352458512469693",
language = "English (US)",
volume = "19",
pages = "891--895",
journal = "Multiple Sclerosis",
issn = "1352-4585",
publisher = "SAGE Publications Ltd",
number = "7",

}

TY - JOUR

T1 - Antibody response to common viruses and human leukocyte antigen-DRB1 in pediatric multiple sclerosis

AU - Waubant, Emmanuelle

AU - Mowry, Ellen Mahar

AU - Krupp, Lauren

AU - Chitnis, Tanuja

AU - Yeh, E. Ann

AU - Kuntz, Nancy

AU - Ness, Jayne

AU - Belman, Anita

AU - Milazzo, Maria

AU - Gorman, Mark

AU - Weinstock-Guttman, Bianca

AU - Rodriguez, Moses

AU - James, Judith A.

PY - 2013

Y1 - 2013

N2 - Background: As remote infections with common herpes viruses are associated with modulation of the risk of multiple sclerosis (MS), we hypothesized that antibody concentrations against these viruses may further modify risk. As many common viruses are first encountered during childhood, pediatric MS offer a unique opportunity to investigate more closely their influence on susceptibility. Our aim was to determine if MS patients who were positive for these viruses had higher levels of antibodies to these viruses. We also assessed whether human leukocyte antigen (HLA)-DRB1*1501 genotype influenced viral antibody levels. Methods: Antibody response levels toward Epstein Barr virus (EBV), cytomegalovirus (CMV), and herpes simplex virus (HSV)-1, and HLA-DRB1*1501 status were determined in pediatric MS patients (n=189) and controls (n=38). Multivariate analyses were used, adjusted for age, gender, race, ethnicity and use of disease-modifying therapies. Results: The antibody concentrations against EBV (Epstein-Barr nuclear antigen 1 (EBNA-1), viral capsid antigen (VCA) and early antigen (EA)), CMV and HSV-1 were similar between pediatric MS patients and controls positive for seroconversion against the virus of interest. EBNA-1 humoral responses were higher in HLA-DRB1 positive individuals (p=0.005) whereas other viral humoral responses were similar in HLA-DRB1 positive and negative individuals. Conclusion: Among those positive for EBNA-1, MS patients did not have higher levels of antibody response to EBNA- 1: however, titers for EBNA-1 were higher in those who were HLA-DRB1 positive. This suggests that genotype might influence the humoral response to EBV. Whether other genotypes influence antibody response to other viruses remains to be determined.

AB - Background: As remote infections with common herpes viruses are associated with modulation of the risk of multiple sclerosis (MS), we hypothesized that antibody concentrations against these viruses may further modify risk. As many common viruses are first encountered during childhood, pediatric MS offer a unique opportunity to investigate more closely their influence on susceptibility. Our aim was to determine if MS patients who were positive for these viruses had higher levels of antibodies to these viruses. We also assessed whether human leukocyte antigen (HLA)-DRB1*1501 genotype influenced viral antibody levels. Methods: Antibody response levels toward Epstein Barr virus (EBV), cytomegalovirus (CMV), and herpes simplex virus (HSV)-1, and HLA-DRB1*1501 status were determined in pediatric MS patients (n=189) and controls (n=38). Multivariate analyses were used, adjusted for age, gender, race, ethnicity and use of disease-modifying therapies. Results: The antibody concentrations against EBV (Epstein-Barr nuclear antigen 1 (EBNA-1), viral capsid antigen (VCA) and early antigen (EA)), CMV and HSV-1 were similar between pediatric MS patients and controls positive for seroconversion against the virus of interest. EBNA-1 humoral responses were higher in HLA-DRB1 positive individuals (p=0.005) whereas other viral humoral responses were similar in HLA-DRB1 positive and negative individuals. Conclusion: Among those positive for EBNA-1, MS patients did not have higher levels of antibody response to EBNA- 1: however, titers for EBNA-1 were higher in those who were HLA-DRB1 positive. This suggests that genotype might influence the humoral response to EBV. Whether other genotypes influence antibody response to other viruses remains to be determined.

KW - cytomegalovirus

KW - DRB1

KW - Epstein-Barr virus

KW - gene-environment interaction

KW - herpes simplex virus-1

KW - Multiple sclerosis susceptibility

KW - pediatric multiple sclerosis

KW - risk factors

UR - http://www.scopus.com/inward/record.url?scp=84878367638&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84878367638&partnerID=8YFLogxK

U2 - 10.1177/1352458512469693

DO - 10.1177/1352458512469693

M3 - Article

C2 - 23232601

AN - SCOPUS:84878367638

VL - 19

SP - 891

EP - 895

JO - Multiple Sclerosis

JF - Multiple Sclerosis

SN - 1352-4585

IS - 7

ER -