TY - JOUR
T1 - Antibody Recognition of SIVmac Envelope Peptides in Plasma from Macaques Experimentally Infected with SIV/Mne
AU - Shafferman, Avigdor
AU - Layne, Arnold
AU - Sadoff, Jerald
AU - Burke, Donald S.
AU - Morton, William R.
AU - Benveniste, Raoul E.
PY - 1989/6
Y1 - 1989/6
N2 - Four stretches of amino acid sequences encoded in conserved HIV-1 env domains and four parallel regions of the SIVmac env (two from gp120 and two from gp41/p32E) were fused to the NH2 terminus of β-galactosidase by recombinant DNA techniques and used to analyze sera from three macaque species experimentally infected with SIV/Mne. All SIVmac env sequences were recognized by sera from the SIV/Mne-inoculated macaques. Western blot analysis performed with whole SIV/Mne, SIVmac, SIVagm, and HIV-1 antigens and sera from SIV/Mne-infected macaques also demonstrates that SIV/Mne is immunologically more closely related to SIVmac than to SIVagm or to HIV-1. Antibody levels to the gp120 NH2-terminal SIV-88 epitope appear to decrease in the infected Macaca nemestrina with progression of disease, as was also reported (see Ref. 16) for the parallel HIV-1 epitope in HIV-1-infected individuals. Sera from all infected macaques reacted with the p32E-SIV-582 epitope (EKYLEDQAQLNAWGCAFRQVC). High titers to this immunodominant epitope could be detected at least 9 weeks postinfection and at a time when primarily the p28 and p32E antibodies were detectable in Western blots performed with whole disrupted SIV/Mne virus. In the majority of animals, antibody titers of 1:100,000 to SIV-582 develop during the infection and persist until death. Antibody responses to the SIV env epitopes in SIV/Mne-infected macaques thus resemble in many aspects (prevalence and immunogenicity) those observed previously (Ref. 16) for the corresponding HIV-1 env epitopes in HIV-1-infected humans.
AB - Four stretches of amino acid sequences encoded in conserved HIV-1 env domains and four parallel regions of the SIVmac env (two from gp120 and two from gp41/p32E) were fused to the NH2 terminus of β-galactosidase by recombinant DNA techniques and used to analyze sera from three macaque species experimentally infected with SIV/Mne. All SIVmac env sequences were recognized by sera from the SIV/Mne-inoculated macaques. Western blot analysis performed with whole SIV/Mne, SIVmac, SIVagm, and HIV-1 antigens and sera from SIV/Mne-infected macaques also demonstrates that SIV/Mne is immunologically more closely related to SIVmac than to SIVagm or to HIV-1. Antibody levels to the gp120 NH2-terminal SIV-88 epitope appear to decrease in the infected Macaca nemestrina with progression of disease, as was also reported (see Ref. 16) for the parallel HIV-1 epitope in HIV-1-infected individuals. Sera from all infected macaques reacted with the p32E-SIV-582 epitope (EKYLEDQAQLNAWGCAFRQVC). High titers to this immunodominant epitope could be detected at least 9 weeks postinfection and at a time when primarily the p28 and p32E antibodies were detectable in Western blots performed with whole disrupted SIV/Mne virus. In the majority of animals, antibody titers of 1:100,000 to SIV-582 develop during the infection and persist until death. Antibody responses to the SIV env epitopes in SIV/Mne-infected macaques thus resemble in many aspects (prevalence and immunogenicity) those observed previously (Ref. 16) for the corresponding HIV-1 env epitopes in HIV-1-infected humans.
UR - http://www.scopus.com/inward/record.url?scp=0024307932&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0024307932&partnerID=8YFLogxK
U2 - 10.1089/aid.1989.5.327
DO - 10.1089/aid.1989.5.327
M3 - Article
C2 - 2543433
AN - SCOPUS:0024307932
SN - 0889-2229
VL - 5
SP - 327
EP - 336
JO - AIDS research and human retroviruses
JF - AIDS research and human retroviruses
IS - 3
ER -