Antibody Maturation and Viral Diversification in HIV-Infected Women

Maria M. James, Oliver B. Laeyendecker, Jin Sun, Donald R. Hoover, Caroline E. Mullis, Matthew M. Cousins, Thomas Coates, Richard D Moore, Gabor D Kelen, Mary Glenn Fowler, Johnstone J. Kumwenda, Lynne M. Mofenson, Newton I. Kumwenda, Taha E Taha, Susan Eshleman

Research output: Contribution to journalArticle

Abstract

Introduction: The Post-exposure Prophylaxis in Infants (PEPI)-Malawi trial evaluated infant antiretroviral regimens for prevention of post-natal HIV transmission. A multi-assay algorithm (MAA) that includes the BED capture immunoassay, an avidity assay, CD4 cell count, and viral load was used to identify women who were vs. were not recently infected at the time of enrollment (MAA recent, N = 73; MAA non-recent, N = 2,488); a subset of the women in the MAA non-recent group known to have been HIV infected for at least 2 years before enrollment (known non-recent, N = 54). Antibody maturation and viral diversification were examined in these women. Methods: Samples collected at enrollment (N = 2,561) and 12-24 months later (N = 1,306) were available for serologic analysis using the BED and avidity assays. A subset of those samples was used for analysis of viral diversity, which was performed using a high resolution melting (HRM) diversity assay. Viral diversity analysis was performed using all available samples from women in the MAA recent group (61 enrollment samples, 38 follow-up samples) and the known non-recent group (43 enrollment samples, 22 follow-up samples). Diversity data from PEPI-Malawi were also compared to similar data from 169 adults in the United States (US) with known recent infection (N = 102) and known non-recent infection (N = 67). Results: In PEPI-Malawi, results from the BED and avidity assays increased over time in the MAA recent group, but did not change significantly in the MAA non-recent group. At enrollment, HIV diversity was lower in the MAA recent group than in the known non-recent group. HRM diversity assay results from women in PEPI-Malawi were similar to those from adults in the US with known duration of HIV infection. Conclusions: Antibody maturation and HIV diversification patterns in African women provide additional support for use of the MAA to identify populations with recent HIV infection.

Original languageEnglish (US)
Article numbere57350
JournalPLoS One
Volume8
Issue number2
DOIs
StatePublished - Feb 27 2013

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Viral Antibodies
Assays
HIV
Post-Exposure Prophylaxis
antibodies
Antibodies
Malawi
assays
disease control
Freezing
HIV Infections
sampling
HIV infections
melting
HIV Antibodies
CD4 Lymphocyte Count
Melting
Infection
Viral Load
Immunoassay

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

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Antibody Maturation and Viral Diversification in HIV-Infected Women. / James, Maria M.; Laeyendecker, Oliver B.; Sun, Jin; Hoover, Donald R.; Mullis, Caroline E.; Cousins, Matthew M.; Coates, Thomas; Moore, Richard D; Kelen, Gabor D; Fowler, Mary Glenn; Kumwenda, Johnstone J.; Mofenson, Lynne M.; Kumwenda, Newton I.; Taha, Taha E; Eshleman, Susan.

In: PLoS One, Vol. 8, No. 2, e57350, 27.02.2013.

Research output: Contribution to journalArticle

James, MM, Laeyendecker, OB, Sun, J, Hoover, DR, Mullis, CE, Cousins, MM, Coates, T, Moore, RD, Kelen, GD, Fowler, MG, Kumwenda, JJ, Mofenson, LM, Kumwenda, NI, Taha, TE & Eshleman, S 2013, 'Antibody Maturation and Viral Diversification in HIV-Infected Women', PLoS One, vol. 8, no. 2, e57350. https://doi.org/10.1371/journal.pone.0057350
James, Maria M. ; Laeyendecker, Oliver B. ; Sun, Jin ; Hoover, Donald R. ; Mullis, Caroline E. ; Cousins, Matthew M. ; Coates, Thomas ; Moore, Richard D ; Kelen, Gabor D ; Fowler, Mary Glenn ; Kumwenda, Johnstone J. ; Mofenson, Lynne M. ; Kumwenda, Newton I. ; Taha, Taha E ; Eshleman, Susan. / Antibody Maturation and Viral Diversification in HIV-Infected Women. In: PLoS One. 2013 ; Vol. 8, No. 2.
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AU - Cousins, Matthew M.

AU - Coates, Thomas

AU - Moore, Richard D

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N2 - Introduction: The Post-exposure Prophylaxis in Infants (PEPI)-Malawi trial evaluated infant antiretroviral regimens for prevention of post-natal HIV transmission. A multi-assay algorithm (MAA) that includes the BED capture immunoassay, an avidity assay, CD4 cell count, and viral load was used to identify women who were vs. were not recently infected at the time of enrollment (MAA recent, N = 73; MAA non-recent, N = 2,488); a subset of the women in the MAA non-recent group known to have been HIV infected for at least 2 years before enrollment (known non-recent, N = 54). Antibody maturation and viral diversification were examined in these women. Methods: Samples collected at enrollment (N = 2,561) and 12-24 months later (N = 1,306) were available for serologic analysis using the BED and avidity assays. A subset of those samples was used for analysis of viral diversity, which was performed using a high resolution melting (HRM) diversity assay. Viral diversity analysis was performed using all available samples from women in the MAA recent group (61 enrollment samples, 38 follow-up samples) and the known non-recent group (43 enrollment samples, 22 follow-up samples). Diversity data from PEPI-Malawi were also compared to similar data from 169 adults in the United States (US) with known recent infection (N = 102) and known non-recent infection (N = 67). Results: In PEPI-Malawi, results from the BED and avidity assays increased over time in the MAA recent group, but did not change significantly in the MAA non-recent group. At enrollment, HIV diversity was lower in the MAA recent group than in the known non-recent group. HRM diversity assay results from women in PEPI-Malawi were similar to those from adults in the US with known duration of HIV infection. Conclusions: Antibody maturation and HIV diversification patterns in African women provide additional support for use of the MAA to identify populations with recent HIV infection.

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