Antibody-dependent cell-mediated cytotoxicity can protect PBMC from infection by cell-associated HIV-1

James E.K. Hildreth, Richard Hampton, Neal A. Halsey

Research output: Contribution to journalArticle

Abstract

We have developed a novel in vitro assay system to study the role of antibody-dependent cell-mediated cytotoxicity (ADCC) in protection against HIV-1 infection by cell-associated virus. HIV-1-infected NK-resistant cells are mixed with specific antibody and unstimulated PBMC and ADCC is allowed to occur over several hours. The PBMC are then activated and cultured to allow virus replication in newly infected T cells. To ensure that ADCC is the only mechanism by which protection could occur we have used haptenated (TNP) infected cells and anti-hapten antibody. Anti-hapten sera completely protected PBMC from infection by haptenated HIV-1-infected cells in ADCC protection assays. F(ab')2 fragments of anti-hapten IgG showed no protection, confirming that ADCC was responsible for protection by anti- hapten IgG. PCR analysis for HIV-1 DNA confirmed the elimination of infected cells. We believe this to be the first direct demonstration that ADCC alone can protect PBMC from infection by cell-associated HIV-1.

Original languageEnglish (US)
Pages (from-to)203-212
Number of pages10
JournalClinical Immunology
Volume90
Issue number2
DOIs
StatePublished - Feb 1999

Keywords

  • ADCC
  • AIDS
  • Antibody
  • HIV
  • Virus neutralization

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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