Antibody-dependent cell-mediated cytotoxicity can protect PBMC from infection by cell-associated HIV-1

James E K Hildreth, Richard Hampton, Neal A Halsey

Research output: Contribution to journalArticle

Abstract

We have developed a novel in vitro assay system to study the role of antibody-dependent cell-mediated cytotoxicity (ADCC) in protection against HIV-1 infection by cell-associated virus. HIV-1-infected NK-resistant cells are mixed with specific antibody and unstimulated PBMC and ADCC is allowed to occur over several hours. The PBMC are then activated and cultured to allow virus replication in newly infected T cells. To ensure that ADCC is the only mechanism by which protection could occur we have used haptenated (TNP) infected cells and anti-hapten antibody. Anti-hapten sera completely protected PBMC from infection by haptenated HIV-1-infected cells in ADCC protection assays. F(ab')2 fragments of anti-hapten IgG showed no protection, confirming that ADCC was responsible for protection by anti- hapten IgG. PCR analysis for HIV-1 DNA confirmed the elimination of infected cells. We believe this to be the first direct demonstration that ADCC alone can protect PBMC from infection by cell-associated HIV-1.

Original languageEnglish (US)
Pages (from-to)203-212
Number of pages10
JournalClinical Immunology
Volume90
Issue number2
DOIs
StatePublished - Feb 1999

Fingerprint

Antibody-Dependent Cell Cytotoxicity
HIV-1
Haptens
Infection
Satellite Viruses
Virus Replication
Natural Killer Cells
HIV Infections
Anti-Idiotypic Antibodies
T-Lymphocytes
Polymerase Chain Reaction
Antibodies
DNA
Serum

Keywords

  • ADCC
  • AIDS
  • Antibody
  • HIV
  • Virus neutralization

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Pathology and Forensic Medicine

Cite this

Antibody-dependent cell-mediated cytotoxicity can protect PBMC from infection by cell-associated HIV-1. / Hildreth, James E K; Hampton, Richard; Halsey, Neal A.

In: Clinical Immunology, Vol. 90, No. 2, 02.1999, p. 203-212.

Research output: Contribution to journalArticle

@article{f17cf0425e6e4062b4d2dbd5dc722f2f,
title = "Antibody-dependent cell-mediated cytotoxicity can protect PBMC from infection by cell-associated HIV-1",
abstract = "We have developed a novel in vitro assay system to study the role of antibody-dependent cell-mediated cytotoxicity (ADCC) in protection against HIV-1 infection by cell-associated virus. HIV-1-infected NK-resistant cells are mixed with specific antibody and unstimulated PBMC and ADCC is allowed to occur over several hours. The PBMC are then activated and cultured to allow virus replication in newly infected T cells. To ensure that ADCC is the only mechanism by which protection could occur we have used haptenated (TNP) infected cells and anti-hapten antibody. Anti-hapten sera completely protected PBMC from infection by haptenated HIV-1-infected cells in ADCC protection assays. F(ab')2 fragments of anti-hapten IgG showed no protection, confirming that ADCC was responsible for protection by anti- hapten IgG. PCR analysis for HIV-1 DNA confirmed the elimination of infected cells. We believe this to be the first direct demonstration that ADCC alone can protect PBMC from infection by cell-associated HIV-1.",
keywords = "ADCC, AIDS, Antibody, HIV, Virus neutralization",
author = "Hildreth, {James E K} and Richard Hampton and Halsey, {Neal A}",
year = "1999",
month = "2",
doi = "10.1006/clim.1998.4624",
language = "English (US)",
volume = "90",
pages = "203--212",
journal = "Clinical Immunology",
issn = "1521-6616",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Antibody-dependent cell-mediated cytotoxicity can protect PBMC from infection by cell-associated HIV-1

AU - Hildreth, James E K

AU - Hampton, Richard

AU - Halsey, Neal A

PY - 1999/2

Y1 - 1999/2

N2 - We have developed a novel in vitro assay system to study the role of antibody-dependent cell-mediated cytotoxicity (ADCC) in protection against HIV-1 infection by cell-associated virus. HIV-1-infected NK-resistant cells are mixed with specific antibody and unstimulated PBMC and ADCC is allowed to occur over several hours. The PBMC are then activated and cultured to allow virus replication in newly infected T cells. To ensure that ADCC is the only mechanism by which protection could occur we have used haptenated (TNP) infected cells and anti-hapten antibody. Anti-hapten sera completely protected PBMC from infection by haptenated HIV-1-infected cells in ADCC protection assays. F(ab')2 fragments of anti-hapten IgG showed no protection, confirming that ADCC was responsible for protection by anti- hapten IgG. PCR analysis for HIV-1 DNA confirmed the elimination of infected cells. We believe this to be the first direct demonstration that ADCC alone can protect PBMC from infection by cell-associated HIV-1.

AB - We have developed a novel in vitro assay system to study the role of antibody-dependent cell-mediated cytotoxicity (ADCC) in protection against HIV-1 infection by cell-associated virus. HIV-1-infected NK-resistant cells are mixed with specific antibody and unstimulated PBMC and ADCC is allowed to occur over several hours. The PBMC are then activated and cultured to allow virus replication in newly infected T cells. To ensure that ADCC is the only mechanism by which protection could occur we have used haptenated (TNP) infected cells and anti-hapten antibody. Anti-hapten sera completely protected PBMC from infection by haptenated HIV-1-infected cells in ADCC protection assays. F(ab')2 fragments of anti-hapten IgG showed no protection, confirming that ADCC was responsible for protection by anti- hapten IgG. PCR analysis for HIV-1 DNA confirmed the elimination of infected cells. We believe this to be the first direct demonstration that ADCC alone can protect PBMC from infection by cell-associated HIV-1.

KW - ADCC

KW - AIDS

KW - Antibody

KW - HIV

KW - Virus neutralization

UR - http://www.scopus.com/inward/record.url?scp=0032946219&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032946219&partnerID=8YFLogxK

U2 - 10.1006/clim.1998.4624

DO - 10.1006/clim.1998.4624

M3 - Article

C2 - 10080832

AN - SCOPUS:0032946219

VL - 90

SP - 203

EP - 212

JO - Clinical Immunology

JF - Clinical Immunology

SN - 1521-6616

IS - 2

ER -