Antibody Banding Patterns of the Enzyme-Linked Immunoelectrotransfer Blot and Brain Imaging Findings in Patients with Neurocysticercosis

Gianfranco Arroyo, Silvia Rodriguez, Andres G. Lescano, Karen A. Alroy, Javier A. Bustos, Saul Santivañez, E. Javier Pretell, Hector H. Garcia, Robert H Gilman, Armandoe Gonzalez, Victor C.W. Tsang, Isidro Gonzalez, Herbert Saavedra, Manuel Martinez, Manuel Alvarado, Manuela Verastegui, Mirko Zimic, Holger Mayta, Cristina Guerra, Yesenia CastilloYagahira Castro, Maria T. Lopez, Cesar M. Gavidia, Luis Gomez, Luz M. Moyano, Ricardo Gamboa, Claudio Muro, Percy Vilchez, Theodore E. Nash, Siddhartha Mahanty, John Noh, Sukwan Handali, Jon Friedland

Research output: Contribution to journalArticle

Abstract

Background The enzyme-linked immunoelectrotransfer blot (EITB) assay is the reference serological test for neurocysticercosis (NCC). A positive result on EITB does not always correlate with the presence of active infections in the central nervous system (CNS), and patients with a single viable brain cyst may be EITB negative. Nonetheless, EITB antibody banding patterns appears to be related with the expression of 3 protein families of Taenia solium, and in turn with the characteristics of NCC in the CNS (type, stage, and burden of viable cysts). Methods We evaluated EITB antibody banding patterns and brain imaging findings of 548 NCC cases. Similar banding patterns were grouped into homogeneous classes using latent class analysis. The association between classes and brain imaging findings was assessed. Results Four classes were identified. Class 1 (patients negative or only positive to the GP50 band, related to the protein family of the same name) was associated with nonviable or single viable parenchymal cysticerci; class 2 (patients positive to bands GP42-39 and GP24, related to the T24-42 protein family, with or without anti-GP50 antibodies) was associated with intraparenchymal viable and nonviable infections; classes 3 and 4 (positive to GP50, GP42-39, and GP24 but also responding to low molecular weight bands GP21, GP18, GP14, and GP13, related to the 8 kDa protein family) were associated with extraparenchymal and intraparenchymal multiple viable cysticerci. Conclusions EITB antibody banding patterns correlate with brain imaging findings and complement imaging information for the diagnosis of NCC and for staging NCC patients.

Original languageEnglish (US)
Pages (from-to)282-288
Number of pages7
JournalClinical Infectious Diseases
Volume66
Issue number2
DOIs
StatePublished - Jan 15 2018
Externally publishedYes

Fingerprint

Neurocysticercosis
Neuroimaging
Antibodies
Enzymes
Cysticercus
Cysts
Proteins
Taenia solium
Central Nervous System Infections
Serologic Tests
Names
Anti-Idiotypic Antibodies
Central Nervous System
Molecular Weight
Brain
Infection
brain synaptic membrane glycoprotein gp 50

Keywords

  • EITB
  • epilepsy
  • neurocysticercosis
  • Peru
  • Taenia solium

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

Cite this

Arroyo, G., Rodriguez, S., Lescano, A. G., Alroy, K. A., Bustos, J. A., Santivañez, S., ... Friedland, J. (2018). Antibody Banding Patterns of the Enzyme-Linked Immunoelectrotransfer Blot and Brain Imaging Findings in Patients with Neurocysticercosis. Clinical Infectious Diseases, 66(2), 282-288. https://doi.org/10.1093/cid/cix774

Antibody Banding Patterns of the Enzyme-Linked Immunoelectrotransfer Blot and Brain Imaging Findings in Patients with Neurocysticercosis. / Arroyo, Gianfranco; Rodriguez, Silvia; Lescano, Andres G.; Alroy, Karen A.; Bustos, Javier A.; Santivañez, Saul; Pretell, E. Javier; Garcia, Hector H.; Gilman, Robert H; Gonzalez, Armandoe; Tsang, Victor C.W.; Gonzalez, Isidro; Saavedra, Herbert; Martinez, Manuel; Alvarado, Manuel; Verastegui, Manuela; Zimic, Mirko; Mayta, Holger; Guerra, Cristina; Castillo, Yesenia; Castro, Yagahira; Lopez, Maria T.; Gavidia, Cesar M.; Gomez, Luis; Moyano, Luz M.; Gamboa, Ricardo; Muro, Claudio; Vilchez, Percy; Nash, Theodore E.; Mahanty, Siddhartha; Noh, John; Handali, Sukwan; Friedland, Jon.

In: Clinical Infectious Diseases, Vol. 66, No. 2, 15.01.2018, p. 282-288.

Research output: Contribution to journalArticle

Arroyo, G, Rodriguez, S, Lescano, AG, Alroy, KA, Bustos, JA, Santivañez, S, Pretell, EJ, Garcia, HH, Gilman, RH, Gonzalez, A, Tsang, VCW, Gonzalez, I, Saavedra, H, Martinez, M, Alvarado, M, Verastegui, M, Zimic, M, Mayta, H, Guerra, C, Castillo, Y, Castro, Y, Lopez, MT, Gavidia, CM, Gomez, L, Moyano, LM, Gamboa, R, Muro, C, Vilchez, P, Nash, TE, Mahanty, S, Noh, J, Handali, S & Friedland, J 2018, 'Antibody Banding Patterns of the Enzyme-Linked Immunoelectrotransfer Blot and Brain Imaging Findings in Patients with Neurocysticercosis', Clinical Infectious Diseases, vol. 66, no. 2, pp. 282-288. https://doi.org/10.1093/cid/cix774
Arroyo, Gianfranco ; Rodriguez, Silvia ; Lescano, Andres G. ; Alroy, Karen A. ; Bustos, Javier A. ; Santivañez, Saul ; Pretell, E. Javier ; Garcia, Hector H. ; Gilman, Robert H ; Gonzalez, Armandoe ; Tsang, Victor C.W. ; Gonzalez, Isidro ; Saavedra, Herbert ; Martinez, Manuel ; Alvarado, Manuel ; Verastegui, Manuela ; Zimic, Mirko ; Mayta, Holger ; Guerra, Cristina ; Castillo, Yesenia ; Castro, Yagahira ; Lopez, Maria T. ; Gavidia, Cesar M. ; Gomez, Luis ; Moyano, Luz M. ; Gamboa, Ricardo ; Muro, Claudio ; Vilchez, Percy ; Nash, Theodore E. ; Mahanty, Siddhartha ; Noh, John ; Handali, Sukwan ; Friedland, Jon. / Antibody Banding Patterns of the Enzyme-Linked Immunoelectrotransfer Blot and Brain Imaging Findings in Patients with Neurocysticercosis. In: Clinical Infectious Diseases. 2018 ; Vol. 66, No. 2. pp. 282-288.
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abstract = "Background The enzyme-linked immunoelectrotransfer blot (EITB) assay is the reference serological test for neurocysticercosis (NCC). A positive result on EITB does not always correlate with the presence of active infections in the central nervous system (CNS), and patients with a single viable brain cyst may be EITB negative. Nonetheless, EITB antibody banding patterns appears to be related with the expression of 3 protein families of Taenia solium, and in turn with the characteristics of NCC in the CNS (type, stage, and burden of viable cysts). Methods We evaluated EITB antibody banding patterns and brain imaging findings of 548 NCC cases. Similar banding patterns were grouped into homogeneous classes using latent class analysis. The association between classes and brain imaging findings was assessed. Results Four classes were identified. Class 1 (patients negative or only positive to the GP50 band, related to the protein family of the same name) was associated with nonviable or single viable parenchymal cysticerci; class 2 (patients positive to bands GP42-39 and GP24, related to the T24-42 protein family, with or without anti-GP50 antibodies) was associated with intraparenchymal viable and nonviable infections; classes 3 and 4 (positive to GP50, GP42-39, and GP24 but also responding to low molecular weight bands GP21, GP18, GP14, and GP13, related to the 8 kDa protein family) were associated with extraparenchymal and intraparenchymal multiple viable cysticerci. Conclusions EITB antibody banding patterns correlate with brain imaging findings and complement imaging information for the diagnosis of NCC and for staging NCC patients.",
keywords = "EITB, epilepsy, neurocysticercosis, Peru, Taenia solium",
author = "Gianfranco Arroyo and Silvia Rodriguez and Lescano, {Andres G.} and Alroy, {Karen A.} and Bustos, {Javier A.} and Saul Santiva{\~n}ez and Pretell, {E. Javier} and Garcia, {Hector H.} and Gilman, {Robert H} and Armandoe Gonzalez and Tsang, {Victor C.W.} and Isidro Gonzalez and Herbert Saavedra and Manuel Martinez and Manuel Alvarado and Manuela Verastegui and Mirko Zimic and Holger Mayta and Cristina Guerra and Yesenia Castillo and Yagahira Castro and Lopez, {Maria T.} and Gavidia, {Cesar M.} and Luis Gomez and Moyano, {Luz M.} and Ricardo Gamboa and Claudio Muro and Percy Vilchez and Nash, {Theodore E.} and Siddhartha Mahanty and John Noh and Sukwan Handali and Jon Friedland",
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T1 - Antibody Banding Patterns of the Enzyme-Linked Immunoelectrotransfer Blot and Brain Imaging Findings in Patients with Neurocysticercosis

AU - Arroyo, Gianfranco

AU - Rodriguez, Silvia

AU - Lescano, Andres G.

AU - Alroy, Karen A.

AU - Bustos, Javier A.

AU - Santivañez, Saul

AU - Pretell, E. Javier

AU - Garcia, Hector H.

AU - Gilman, Robert H

AU - Gonzalez, Armandoe

AU - Tsang, Victor C.W.

AU - Gonzalez, Isidro

AU - Saavedra, Herbert

AU - Martinez, Manuel

AU - Alvarado, Manuel

AU - Verastegui, Manuela

AU - Zimic, Mirko

AU - Mayta, Holger

AU - Guerra, Cristina

AU - Castillo, Yesenia

AU - Castro, Yagahira

AU - Lopez, Maria T.

AU - Gavidia, Cesar M.

AU - Gomez, Luis

AU - Moyano, Luz M.

AU - Gamboa, Ricardo

AU - Muro, Claudio

AU - Vilchez, Percy

AU - Nash, Theodore E.

AU - Mahanty, Siddhartha

AU - Noh, John

AU - Handali, Sukwan

AU - Friedland, Jon

PY - 2018/1/15

Y1 - 2018/1/15

N2 - Background The enzyme-linked immunoelectrotransfer blot (EITB) assay is the reference serological test for neurocysticercosis (NCC). A positive result on EITB does not always correlate with the presence of active infections in the central nervous system (CNS), and patients with a single viable brain cyst may be EITB negative. Nonetheless, EITB antibody banding patterns appears to be related with the expression of 3 protein families of Taenia solium, and in turn with the characteristics of NCC in the CNS (type, stage, and burden of viable cysts). Methods We evaluated EITB antibody banding patterns and brain imaging findings of 548 NCC cases. Similar banding patterns were grouped into homogeneous classes using latent class analysis. The association between classes and brain imaging findings was assessed. Results Four classes were identified. Class 1 (patients negative or only positive to the GP50 band, related to the protein family of the same name) was associated with nonviable or single viable parenchymal cysticerci; class 2 (patients positive to bands GP42-39 and GP24, related to the T24-42 protein family, with or without anti-GP50 antibodies) was associated with intraparenchymal viable and nonviable infections; classes 3 and 4 (positive to GP50, GP42-39, and GP24 but also responding to low molecular weight bands GP21, GP18, GP14, and GP13, related to the 8 kDa protein family) were associated with extraparenchymal and intraparenchymal multiple viable cysticerci. Conclusions EITB antibody banding patterns correlate with brain imaging findings and complement imaging information for the diagnosis of NCC and for staging NCC patients.

AB - Background The enzyme-linked immunoelectrotransfer blot (EITB) assay is the reference serological test for neurocysticercosis (NCC). A positive result on EITB does not always correlate with the presence of active infections in the central nervous system (CNS), and patients with a single viable brain cyst may be EITB negative. Nonetheless, EITB antibody banding patterns appears to be related with the expression of 3 protein families of Taenia solium, and in turn with the characteristics of NCC in the CNS (type, stage, and burden of viable cysts). Methods We evaluated EITB antibody banding patterns and brain imaging findings of 548 NCC cases. Similar banding patterns were grouped into homogeneous classes using latent class analysis. The association between classes and brain imaging findings was assessed. Results Four classes were identified. Class 1 (patients negative or only positive to the GP50 band, related to the protein family of the same name) was associated with nonviable or single viable parenchymal cysticerci; class 2 (patients positive to bands GP42-39 and GP24, related to the T24-42 protein family, with or without anti-GP50 antibodies) was associated with intraparenchymal viable and nonviable infections; classes 3 and 4 (positive to GP50, GP42-39, and GP24 but also responding to low molecular weight bands GP21, GP18, GP14, and GP13, related to the 8 kDa protein family) were associated with extraparenchymal and intraparenchymal multiple viable cysticerci. Conclusions EITB antibody banding patterns correlate with brain imaging findings and complement imaging information for the diagnosis of NCC and for staging NCC patients.

KW - EITB

KW - epilepsy

KW - neurocysticercosis

KW - Peru

KW - Taenia solium

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