TY - JOUR
T1 - Antibody-antigen binding in organic solvents
AU - Russell, Alan J.
AU - Trudel, Laura J.
AU - Skipper, Paul L.
AU - Groopman, John D.
AU - Tannenbaum, Steven R.
AU - Klibanov, Alexander M.
N1 - Funding Information:
Acknowledgements: This work was funded by the U.S. Department of Energy through Contract No. 957381 (ECUT Biocatalysis Project) to A.M.K. A.J.R. is a SERC/NATO postdoctoral fellow.
PY - 1989/1/16
Y1 - 1989/1/16
N2 - We describe, for the first time, the action of antibodies in anhydrous organic solvents. It has been demonstrated that the binding of a hapten, 4-aminobiphenyl, to the immobilized monoclonal antibody 2E11 is strong and specific not only in water but also in a variety of non-aqueous media. Further, the strength of interaction between antibody and hapten has been related to the hydrophobicity of the solvent: the more hydrophobic the solvent, the weaker the protein-ligand interaction.
AB - We describe, for the first time, the action of antibodies in anhydrous organic solvents. It has been demonstrated that the binding of a hapten, 4-aminobiphenyl, to the immobilized monoclonal antibody 2E11 is strong and specific not only in water but also in a variety of non-aqueous media. Further, the strength of interaction between antibody and hapten has been related to the hydrophobicity of the solvent: the more hydrophobic the solvent, the weaker the protein-ligand interaction.
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U2 - 10.1016/S0006-291X(89)80179-2
DO - 10.1016/S0006-291X(89)80179-2
M3 - Article
C2 - 2912456
AN - SCOPUS:0024499741
SN - 0006-291X
VL - 158
SP - 80
EP - 85
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -