Antibody-antigen binding in organic solvents

Alan J. Russell, Laura J. Trudel, Paul L. Skipper, John Davis Groopman, Steven R. Tannenbaum, Alexander M. Klibanov

Research output: Contribution to journalArticle

Abstract

We describe, for the first time, the action of antibodies in anhydrous organic solvents. It has been demonstrated that the binding of a hapten, 4-aminobiphenyl, to the immobilized monoclonal antibody 2E11 is strong and specific not only in water but also in a variety of non-aqueous media. Further, the strength of interaction between antibody and hapten has been related to the hydrophobicity of the solvent: the more hydrophobic the solvent, the weaker the protein-ligand interaction.

Original languageEnglish (US)
Pages (from-to)80-85
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume158
Issue number1
DOIs
StatePublished - Jan 16 1989
Externally publishedYes

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Haptens
Organic solvents
Antigens
Antibodies
Hydrophobicity
Immobilized Antibodies
Monoclonal Antibodies
Ligands
Hydrophobic and Hydrophilic Interactions
Water
Proteins
4-biphenylamine

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Russell, A. J., Trudel, L. J., Skipper, P. L., Groopman, J. D., Tannenbaum, S. R., & Klibanov, A. M. (1989). Antibody-antigen binding in organic solvents. Biochemical and Biophysical Research Communications, 158(1), 80-85. https://doi.org/10.1016/S0006-291X(89)80179-2

Antibody-antigen binding in organic solvents. / Russell, Alan J.; Trudel, Laura J.; Skipper, Paul L.; Groopman, John Davis; Tannenbaum, Steven R.; Klibanov, Alexander M.

In: Biochemical and Biophysical Research Communications, Vol. 158, No. 1, 16.01.1989, p. 80-85.

Research output: Contribution to journalArticle

Russell, AJ, Trudel, LJ, Skipper, PL, Groopman, JD, Tannenbaum, SR & Klibanov, AM 1989, 'Antibody-antigen binding in organic solvents', Biochemical and Biophysical Research Communications, vol. 158, no. 1, pp. 80-85. https://doi.org/10.1016/S0006-291X(89)80179-2
Russell, Alan J. ; Trudel, Laura J. ; Skipper, Paul L. ; Groopman, John Davis ; Tannenbaum, Steven R. ; Klibanov, Alexander M. / Antibody-antigen binding in organic solvents. In: Biochemical and Biophysical Research Communications. 1989 ; Vol. 158, No. 1. pp. 80-85.
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