Antibodies to Trichomonas vaginalis surface glycolipid

F. D. Bastida-Corcuera, B. N. Singh, G. C. Gray, Paul Stamper, M. Davuluri, K. Schlangen, R. R. Corbeil, L. B. Corbeil

Research output: Contribution to journalArticle

Abstract

Background Human trichomoniasis is the most common non-viral sexually transmitted disease, yet immune responses are not well studied. Methods Since the Trichomonas vaginalis lipophosphoglycan (TvLPG) is an important virulence factor, a bank of eight monoclonal antibodies was generated to define the antigen in clinical isolates. The TvLPG-specific antibody response of women who were culture positive (n=33) or negative (n=33) for T vaginalis infection was determined by isotype-specific ELISA. Results The bank of monoclonal antibodies reacted with conserved surface TvLPG epitopes in 27 isolates from pregnant women at their first prenatal visit. Conserved TvLPG epitopes were shown to be surface exposed by immunofluorescence. Sera collected from the same patients at the same time were assayed for specific antibodies. Serum and vaginal secretions from 33 T vaginalis-positive women had statistically higher IgG anti-TvLPG levels than age-matched and race-matched negative controls in the same clinical study ( p<0.01). Vaginal IgA anti-TvLPG levels of the women with trichomoniasis were almost significantly higher than controls (p=0.055). Infected women with normal pregnancies had significantly higher vaginal IgG anti-TvLPG values than infected women with adverse outcomes of pregnancy. Conclusions These antibody responses show that infected women can respond to the conserved TvLPG antigen. Since antibodies to trichomonad surface LPG protect in a bovine model of trichomoniasis, the role of these antibodies in the human disease should be investigated.

Original languageEnglish (US)
Pages (from-to)467-472
Number of pages6
JournalSexually Transmitted Infections
Volume89
Issue number6
DOIs
StatePublished - Sep 1 2013
Externally publishedYes

Fingerprint

Trichomonas vaginalis
Glycolipids
Antibodies
Antibody Formation
Epitopes
Monoclonal Antibodies
Penicillin G Benzathine
Antigens
lipophosphonoglycan
Virulence Factors
Pregnancy Outcome
Sexually Transmitted Diseases
Serum
Fluorescent Antibody Technique
Pregnant Women
Enzyme-Linked Immunosorbent Assay
Pregnancy

ASJC Scopus subject areas

  • Dermatology
  • Infectious Diseases

Cite this

Bastida-Corcuera, F. D., Singh, B. N., Gray, G. C., Stamper, P., Davuluri, M., Schlangen, K., ... Corbeil, L. B. (2013). Antibodies to Trichomonas vaginalis surface glycolipid. Sexually Transmitted Infections, 89(6), 467-472. https://doi.org/10.1136/sextrans-2012-051013

Antibodies to Trichomonas vaginalis surface glycolipid. / Bastida-Corcuera, F. D.; Singh, B. N.; Gray, G. C.; Stamper, Paul; Davuluri, M.; Schlangen, K.; Corbeil, R. R.; Corbeil, L. B.

In: Sexually Transmitted Infections, Vol. 89, No. 6, 01.09.2013, p. 467-472.

Research output: Contribution to journalArticle

Bastida-Corcuera, FD, Singh, BN, Gray, GC, Stamper, P, Davuluri, M, Schlangen, K, Corbeil, RR & Corbeil, LB 2013, 'Antibodies to Trichomonas vaginalis surface glycolipid', Sexually Transmitted Infections, vol. 89, no. 6, pp. 467-472. https://doi.org/10.1136/sextrans-2012-051013
Bastida-Corcuera FD, Singh BN, Gray GC, Stamper P, Davuluri M, Schlangen K et al. Antibodies to Trichomonas vaginalis surface glycolipid. Sexually Transmitted Infections. 2013 Sep 1;89(6):467-472. https://doi.org/10.1136/sextrans-2012-051013
Bastida-Corcuera, F. D. ; Singh, B. N. ; Gray, G. C. ; Stamper, Paul ; Davuluri, M. ; Schlangen, K. ; Corbeil, R. R. ; Corbeil, L. B. / Antibodies to Trichomonas vaginalis surface glycolipid. In: Sexually Transmitted Infections. 2013 ; Vol. 89, No. 6. pp. 467-472.
@article{75854fb476094b549f877ef84e9e7b85,
title = "Antibodies to Trichomonas vaginalis surface glycolipid",
abstract = "Background Human trichomoniasis is the most common non-viral sexually transmitted disease, yet immune responses are not well studied. Methods Since the Trichomonas vaginalis lipophosphoglycan (TvLPG) is an important virulence factor, a bank of eight monoclonal antibodies was generated to define the antigen in clinical isolates. The TvLPG-specific antibody response of women who were culture positive (n=33) or negative (n=33) for T vaginalis infection was determined by isotype-specific ELISA. Results The bank of monoclonal antibodies reacted with conserved surface TvLPG epitopes in 27 isolates from pregnant women at their first prenatal visit. Conserved TvLPG epitopes were shown to be surface exposed by immunofluorescence. Sera collected from the same patients at the same time were assayed for specific antibodies. Serum and vaginal secretions from 33 T vaginalis-positive women had statistically higher IgG anti-TvLPG levels than age-matched and race-matched negative controls in the same clinical study ( p<0.01). Vaginal IgA anti-TvLPG levels of the women with trichomoniasis were almost significantly higher than controls (p=0.055). Infected women with normal pregnancies had significantly higher vaginal IgG anti-TvLPG values than infected women with adverse outcomes of pregnancy. Conclusions These antibody responses show that infected women can respond to the conserved TvLPG antigen. Since antibodies to trichomonad surface LPG protect in a bovine model of trichomoniasis, the role of these antibodies in the human disease should be investigated.",
author = "Bastida-Corcuera, {F. D.} and Singh, {B. N.} and Gray, {G. C.} and Paul Stamper and M. Davuluri and K. Schlangen and Corbeil, {R. R.} and Corbeil, {L. B.}",
year = "2013",
month = "9",
day = "1",
doi = "10.1136/sextrans-2012-051013",
language = "English (US)",
volume = "89",
pages = "467--472",
journal = "Sexually Transmitted Infections",
issn = "1368-4973",
publisher = "BMJ Publishing Group",
number = "6",

}

TY - JOUR

T1 - Antibodies to Trichomonas vaginalis surface glycolipid

AU - Bastida-Corcuera, F. D.

AU - Singh, B. N.

AU - Gray, G. C.

AU - Stamper, Paul

AU - Davuluri, M.

AU - Schlangen, K.

AU - Corbeil, R. R.

AU - Corbeil, L. B.

PY - 2013/9/1

Y1 - 2013/9/1

N2 - Background Human trichomoniasis is the most common non-viral sexually transmitted disease, yet immune responses are not well studied. Methods Since the Trichomonas vaginalis lipophosphoglycan (TvLPG) is an important virulence factor, a bank of eight monoclonal antibodies was generated to define the antigen in clinical isolates. The TvLPG-specific antibody response of women who were culture positive (n=33) or negative (n=33) for T vaginalis infection was determined by isotype-specific ELISA. Results The bank of monoclonal antibodies reacted with conserved surface TvLPG epitopes in 27 isolates from pregnant women at their first prenatal visit. Conserved TvLPG epitopes were shown to be surface exposed by immunofluorescence. Sera collected from the same patients at the same time were assayed for specific antibodies. Serum and vaginal secretions from 33 T vaginalis-positive women had statistically higher IgG anti-TvLPG levels than age-matched and race-matched negative controls in the same clinical study ( p<0.01). Vaginal IgA anti-TvLPG levels of the women with trichomoniasis were almost significantly higher than controls (p=0.055). Infected women with normal pregnancies had significantly higher vaginal IgG anti-TvLPG values than infected women with adverse outcomes of pregnancy. Conclusions These antibody responses show that infected women can respond to the conserved TvLPG antigen. Since antibodies to trichomonad surface LPG protect in a bovine model of trichomoniasis, the role of these antibodies in the human disease should be investigated.

AB - Background Human trichomoniasis is the most common non-viral sexually transmitted disease, yet immune responses are not well studied. Methods Since the Trichomonas vaginalis lipophosphoglycan (TvLPG) is an important virulence factor, a bank of eight monoclonal antibodies was generated to define the antigen in clinical isolates. The TvLPG-specific antibody response of women who were culture positive (n=33) or negative (n=33) for T vaginalis infection was determined by isotype-specific ELISA. Results The bank of monoclonal antibodies reacted with conserved surface TvLPG epitopes in 27 isolates from pregnant women at their first prenatal visit. Conserved TvLPG epitopes were shown to be surface exposed by immunofluorescence. Sera collected from the same patients at the same time were assayed for specific antibodies. Serum and vaginal secretions from 33 T vaginalis-positive women had statistically higher IgG anti-TvLPG levels than age-matched and race-matched negative controls in the same clinical study ( p<0.01). Vaginal IgA anti-TvLPG levels of the women with trichomoniasis were almost significantly higher than controls (p=0.055). Infected women with normal pregnancies had significantly higher vaginal IgG anti-TvLPG values than infected women with adverse outcomes of pregnancy. Conclusions These antibody responses show that infected women can respond to the conserved TvLPG antigen. Since antibodies to trichomonad surface LPG protect in a bovine model of trichomoniasis, the role of these antibodies in the human disease should be investigated.

UR - http://www.scopus.com/inward/record.url?scp=84884534899&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84884534899&partnerID=8YFLogxK

U2 - 10.1136/sextrans-2012-051013

DO - 10.1136/sextrans-2012-051013

M3 - Article

C2 - 23785040

AN - SCOPUS:84884534899

VL - 89

SP - 467

EP - 472

JO - Sexually Transmitted Infections

JF - Sexually Transmitted Infections

SN - 1368-4973

IS - 6

ER -