TY - JOUR
T1 - Antibodies to Recombinant gp160 in Mucosal Secretions and Sera of Persons Infected with HIV-1 and Seronegative Vaccine Recipients
AU - Funkhouser, Ann
AU - Clements, Mary Lou
AU - Slome, Sally
AU - Clayman, Barbara
AU - Viscidi, Raphael
PY - 1993/7
Y1 - 1993/7
N2 - An enzyme immunoassay (EIA) was developed to detect secretory IgA (sIgA) antibodies to HIV-1 envelope glycoprotein, using a mouse monoclonal antibody and a highly purified, baculovirus-expressed recombinant gp160 (rgp160) as antigen. Detection of sIgA was enhanced by prior immunoprecipitation of IgG. IgG and sIgA rgp160 antibodies were measured in parotid saliva and nasal wash samples of 18 HIV-1-seropositive volunteers and 14 HIV-1-seronegative adult volunteers immunized 3 times with HIV-1 IIIB rgp160 vaccine at 1 of 4 dosage levels: 40 μg (N = 3), 80 μg (N = 3), 160 μg (N = 4), and 640 μg (N = 4). We detected rgp160-specific IgG antibody in the nasal wash samples of all HIV-1-seropositive volunteers and 4/8 vaccinees (50%) immunized with the two highest doses of rgp160 vaccine. All infected volunteers tested had rgp160-specific sIgA in their nasal wash samples. None of the vaccinees and very few of infected volunteer specimens had detectable antibody in the parotid saliva samples (5/8 had IgG and 1/8 had sIgA). We also detected IgG antibody to rgp160 in the sera of all infected volunteers and 13/14 vaccineees (93%). With this EIA, sIgA antibody can be measured in mucosal secretions of recipients of appropriate candidate HIV-1 vaccines.
AB - An enzyme immunoassay (EIA) was developed to detect secretory IgA (sIgA) antibodies to HIV-1 envelope glycoprotein, using a mouse monoclonal antibody and a highly purified, baculovirus-expressed recombinant gp160 (rgp160) as antigen. Detection of sIgA was enhanced by prior immunoprecipitation of IgG. IgG and sIgA rgp160 antibodies were measured in parotid saliva and nasal wash samples of 18 HIV-1-seropositive volunteers and 14 HIV-1-seronegative adult volunteers immunized 3 times with HIV-1 IIIB rgp160 vaccine at 1 of 4 dosage levels: 40 μg (N = 3), 80 μg (N = 3), 160 μg (N = 4), and 640 μg (N = 4). We detected rgp160-specific IgG antibody in the nasal wash samples of all HIV-1-seropositive volunteers and 4/8 vaccinees (50%) immunized with the two highest doses of rgp160 vaccine. All infected volunteers tested had rgp160-specific sIgA in their nasal wash samples. None of the vaccinees and very few of infected volunteer specimens had detectable antibody in the parotid saliva samples (5/8 had IgG and 1/8 had sIgA). We also detected IgG antibody to rgp160 in the sera of all infected volunteers and 13/14 vaccineees (93%). With this EIA, sIgA antibody can be measured in mucosal secretions of recipients of appropriate candidate HIV-1 vaccines.
UR - http://www.scopus.com/inward/record.url?scp=0027237806&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027237806&partnerID=8YFLogxK
U2 - 10.1089/aid.1993.9.627
DO - 10.1089/aid.1993.9.627
M3 - Article
C2 - 8369168
AN - SCOPUS:0027237806
SN - 0889-2229
VL - 9
SP - 627
EP - 632
JO - AIDS research and human retroviruses
JF - AIDS research and human retroviruses
IS - 7
ER -