Antibodies to Plasmodium falciparum gamete surface antigens in Papua New Guinea sera

PATRICIA M. GRAVES, RICHARD CARTERS, THOMAS R. BURKOT, ISABELLA A. QUAKYI, NIRBHAY KUMAR

Research output: Contribution to journalArticle

Abstract

Summary Sera from individuals living in malaria endemic areas of Papua New Guinea were tested for their effect on inactivity of Plasmodium falciparum gametocyles grown in culture to Anopheles freeborni mosquitoes. Consistent reduction of infectivity to less than 5% of control was observed with nine out of the 41 sera from the endemic area tested and also with three out of seven sera tested from individuals rarely exposed to malaria infection. Gamete surface antigens recognized by the sera were investigated by immunoprecipitation from 125I surface‐labelled gametes extracted in SDS and Triton X‐100. The main antigens recognized were of the same mol. wt (230, 48 and 45 kD) as those known to be targets of transmission‐blocking monoclonal antibodies. A significant negative correlation was observed between the total ct/min immunoprecipitated from surface‐labelled gametes by the sera and the average number of oocysts per gut observed in membrane feeding experiments with these sera. Spearmann's rank correlation coefficient indicated that suppression of infectivily correlated strongly with the presence of antibodies against the 230 kD protein; there was no significant correlation between suppression and antibodies to the 48/45 kD proteins. The antibody response to the different gamete surface antigens varied greatly in sera from the endemic areas suggesting that individuals respond differently to each gamete antigen.

Original languageEnglish (US)
Pages (from-to)209-218
Number of pages10
JournalParasite Immunology
Volume10
Issue number2
DOIs
StatePublished - Mar 1988

Keywords

  • Papua New Guinea
  • Plasmodium falciparum
  • gamete
  • transmission blocking

ASJC Scopus subject areas

  • Parasitology
  • Immunology

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    GRAVES, PATRICIA. M., CARTERS, RICHARD., BURKOT, THOMAS. R., QUAKYI, ISABELLA. A., & KUMAR, NIRBHAY. (1988). Antibodies to Plasmodium falciparum gamete surface antigens in Papua New Guinea sera. Parasite Immunology, 10(2), 209-218. https://doi.org/10.1111/j.1365-3024.1988.tb00215.x