Antibodies to ICAM-1 protect kidneys in severe ischemic reperfusion injury

Hamid Rabb, Carmelo C. Mendiola, Sabiha R. Saba, John R. Dietz, C. Wayne Smith, Joseph V. Bonventre, German Ramirez

Research output: Contribution to journalArticlepeer-review

Abstract

ICAM-1 has been implicated in the pathophysiology of ischemic-reperfusion injury in a number of organs, but its role in mediating severe ischemic-reperfusion injury in the kidney has not been extensively studied. Uninephrectomized Sprague Dawley rats were pretreated with either control monoclonal antibody (mAb) or mAb to ICAM-1 and subjected to 60 min of renal artery occlusion. The serum creatinine, complete blood count and kidney histo-pathological damage scores (PDS) (Scale:0-4) were assessed prior to and 24 hours after ischemia. Mean serum creatinine (mg/dl) 24 hours after ischemia was significantly decreased in the anti-ICAM-1 group (1.38 ± 0.23, p<0.001) compared to control (2.87 ± 0.34). PDS was also reduced in anti-ICAM-1 (2.55 ± 0.20, p<0.05) group compared to control (3.35 ± 0.30). These data demonstrate that blocking ICAM-1 significantly mitigates severe ischemic acute renal failure, findings which may lead to improved therapy for this condition.

Original languageEnglish (US)
Pages (from-to)67-73
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume211
Issue number1
DOIs
StatePublished - Jun 6 1995
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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