Antibodies to HPV-16 E6 and E7 proteins as markers for HPV-16-associated invasive cervical cancer

Martin Moller, Raphael P Viscidi, Yeping Sun, Eloisa Guerrero, Peter Hill, Farida Shah, F. Xavier Bosch, Nubia Muñoz, Lutz Gissmann, Keerti V. Shah

Research output: Contribution to journalArticle

Abstract

Transforming proteins E6 and E7 of human papillomaviruses (HPVs) are consistently expressed in HPV-associated cervical cancers. In ELISA with four HPV-16 E6-E7 peptides, patients with HPV-16-associated invasive cervical cancer (group 1) had a greater seroreactivity than all other groups, which included patients with HPV-16-associated cervical intraepithelial neoplasia, invasive cervical cancer patients without HPVs, and unaffected controls. A larger proportion of group 1 sera, as compared to sera of all other groups, was reactive with at least one peptide (49% vs 17-27%), and with two or more peptides (22% vs 0-6%). A clear difference between group 1 and all other groups was also found for high ELISA absorbance values to at least one peptide (22% vs 0-8%). This high seroreactivity of group 1 sera was confirmed by a radioimmunoprecipitation assay with in vitro transcribed and translated HPV-16 E7 protein. Sera from 50% of group 1 but only 3% of controls were reactive in this test. Antibodies to HPV-16 E6 and E7 proteins appear to be virus-specific and disease state-specific markers of HPV-associated cervical cancer.

Original languageEnglish (US)
Pages (from-to)508-514
Number of pages7
JournalVirology
Volume187
Issue number2
DOIs
Publication statusPublished - 1992

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ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

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