TY - JOUR
T1 - Antibodies to a single, conserved epitope in anopheles APN1 inhibit universal transmission of plasmodium falciparum and plasmodium vivax malaria
AU - Armistead, Jennifer S.
AU - Morlais, Isabelle
AU - Mathias, Derrick K.
AU - Jardim, Juliette G.
AU - Joy, Jaimy
AU - Fridman, Arthur
AU - Finnefrock, Adam C.
AU - Bagchi, Ansu
AU - Plebanski, Magdalena
AU - Scorpio, Diana G.
AU - Churcher, Thomas S.
AU - Borg, Natalie A.
AU - Sattabongkot, Jetsumon
AU - Dinglasana, Rhoel R.
PY - 2014/2
Y1 - 2014/2
N2 - Malaria transmission-blocking vaccines (TBVs) represent a promising approach for the elimination and eradication of this disease. AnAPN1 is a lead TBV candidate that targets a surface antigen on the midgut of the obligate vector of the Plasmodium parasite, the Anopheles mosquito. In this study, we demonstrated that antibodies targeting AnAPN1 block transmission of Plasmodium falciparum and Plasmodium vivax across distantly related anopheline species in countries to which malaria is endemic. Using a biochemical and immunological approach, we determined that the mechanism of action for this phenomenon stems from antibody recognition of a single protective epitope on AnAPN1, which we found to be immunogenic in murine and nonhuman primate models and highly conserved among anophelines. These data indicate that AnAPN1 meets the established target product profile for TBVs and suggest a potential key role for an AnAPN1-based panmalaria TBV in the effort to eradicate malaria.
AB - Malaria transmission-blocking vaccines (TBVs) represent a promising approach for the elimination and eradication of this disease. AnAPN1 is a lead TBV candidate that targets a surface antigen on the midgut of the obligate vector of the Plasmodium parasite, the Anopheles mosquito. In this study, we demonstrated that antibodies targeting AnAPN1 block transmission of Plasmodium falciparum and Plasmodium vivax across distantly related anopheline species in countries to which malaria is endemic. Using a biochemical and immunological approach, we determined that the mechanism of action for this phenomenon stems from antibody recognition of a single protective epitope on AnAPN1, which we found to be immunogenic in murine and nonhuman primate models and highly conserved among anophelines. These data indicate that AnAPN1 meets the established target product profile for TBVs and suggest a potential key role for an AnAPN1-based panmalaria TBV in the effort to eradicate malaria.
UR - http://www.scopus.com/inward/record.url?scp=84893008833&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84893008833&partnerID=8YFLogxK
U2 - 10.1128/IAI.01222-13
DO - 10.1128/IAI.01222-13
M3 - Article
C2 - 24478095
AN - SCOPUS:84893008833
SN - 0019-9567
VL - 82
SP - 818
EP - 829
JO - Infection and immunity
JF - Infection and immunity
IS - 2
ER -