Antibodies to 1,2-Naphthoquinone

Robert Ollodart, Noel R. Rose

Research output: Contribution to journalArticle

Abstract

We have prepared an antibody against 1,2-naphthoquinone. This was accomplished by conjugating the quinone to bovine gamma-globulin. The conjugation product showed a visible light absorption peak of 445 mμ. The globulin-quinone antigen was used to produce rabbit antibodies shown to be specific for 1,2-naphthoquinone. The antibodies could be demonstrated by tanned cell hemagglutination and by passive cutaneous anaphylaxis. Quinone conjugated to human serum served as antigen in the former test. In passive cutaneous anaphylaxis, the quinone itself provoked the reaction. However, experimental evidence suggests an in vivo conjugation of the compound with guinea pig serum. The extent of cross-reaction with related naphthoquinone derivatives could be determined quantitatively by inhibition of hemagglutination. The possible value of this type of serological reagent for studying the process of carcinogenesis is discussed.

Original languageEnglish (US)
Pages (from-to)689-695
Number of pages7
JournalCancer Research
Volume22
Issue number6
StatePublished - Jan 1962

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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    Ollodart, R., & Rose, N. R. (1962). Antibodies to 1,2-Naphthoquinone. Cancer Research, 22(6), 689-695.