Abstract
Listeria monocytogenes causes meningitis and encephalitis in humans and crosses the blood-brain barrier by yet unknown mechanisms. The interaction of the bacteria with different types of endothelial cells was recently analyzed, and it was shown that invasion into, but not adhesion to, human brain microvascular endothelial cells (HBMEC) depends on the product of the InlB gene, the surface molecule InlB, which is a member of the internalin multigene family. In the present study we analyzed the role of the medium composition in the interaction of L. monocytogenes with HBMEC, and we show that invasion of HBMEC is strongly inhibited in the presence of adult human serum. The strong inhibitory activity, which is not present in fetal calf serum, does not inhibit uptake by macrophage-like J774 cells but does also inhibit invasion of Caco-2 epithelial cells. The inhibitory component of human serum was identified as being associated with L. monocytogenes-specific antibodies present in the human serum. Human newborn serum (cord serum) shows only a weak inhibitory activity on the invasion of HBMEC by L. monocytogenes.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 95-100 |
| Number of pages | 6 |
| Journal | Infection and Immunity |
| Volume | 71 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 1 2003 |
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ASJC Scopus subject areas
- Immunology
Cite this
Antibodies present in normal human serum inhibit invasion of human brain microvascular endothelial cells by Listeria monocytogenes. / Hertzig, Tobias; Weber, Martin; Greiffenberg, Lars; Holthausen, Britta Schulte; Goebel, Werner; Kim, Kwang Sik; Kuhn, Michael.
In: Infection and Immunity, Vol. 71, No. 1, 01.01.2003, p. 95-100.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Antibodies present in normal human serum inhibit invasion of human brain microvascular endothelial cells by Listeria monocytogenes
AU - Hertzig, Tobias
AU - Weber, Martin
AU - Greiffenberg, Lars
AU - Holthausen, Britta Schulte
AU - Goebel, Werner
AU - Kim, Kwang Sik
AU - Kuhn, Michael
PY - 2003/1/1
Y1 - 2003/1/1
N2 - Listeria monocytogenes causes meningitis and encephalitis in humans and crosses the blood-brain barrier by yet unknown mechanisms. The interaction of the bacteria with different types of endothelial cells was recently analyzed, and it was shown that invasion into, but not adhesion to, human brain microvascular endothelial cells (HBMEC) depends on the product of the InlB gene, the surface molecule InlB, which is a member of the internalin multigene family. In the present study we analyzed the role of the medium composition in the interaction of L. monocytogenes with HBMEC, and we show that invasion of HBMEC is strongly inhibited in the presence of adult human serum. The strong inhibitory activity, which is not present in fetal calf serum, does not inhibit uptake by macrophage-like J774 cells but does also inhibit invasion of Caco-2 epithelial cells. The inhibitory component of human serum was identified as being associated with L. monocytogenes-specific antibodies present in the human serum. Human newborn serum (cord serum) shows only a weak inhibitory activity on the invasion of HBMEC by L. monocytogenes.
AB - Listeria monocytogenes causes meningitis and encephalitis in humans and crosses the blood-brain barrier by yet unknown mechanisms. The interaction of the bacteria with different types of endothelial cells was recently analyzed, and it was shown that invasion into, but not adhesion to, human brain microvascular endothelial cells (HBMEC) depends on the product of the InlB gene, the surface molecule InlB, which is a member of the internalin multigene family. In the present study we analyzed the role of the medium composition in the interaction of L. monocytogenes with HBMEC, and we show that invasion of HBMEC is strongly inhibited in the presence of adult human serum. The strong inhibitory activity, which is not present in fetal calf serum, does not inhibit uptake by macrophage-like J774 cells but does also inhibit invasion of Caco-2 epithelial cells. The inhibitory component of human serum was identified as being associated with L. monocytogenes-specific antibodies present in the human serum. Human newborn serum (cord serum) shows only a weak inhibitory activity on the invasion of HBMEC by L. monocytogenes.
UR - http://www.scopus.com/inward/record.url?scp=0037222413&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0037222413&partnerID=8YFLogxK
U2 - 10.1128/IAI.71.1.95-100.2003
DO - 10.1128/IAI.71.1.95-100.2003
M3 - Article
C2 - 12496153
AN - SCOPUS:0037222413
VL - 71
SP - 95
EP - 100
JO - Infection and Immunity
JF - Infection and Immunity
SN - 0019-9567
IS - 1
ER -