Antibiotic resistance genes in multidrug-resistant Enterococcus spp. and Streptococcus spp. recovered from the indoor air of a large-scale swine-feeding operation

A. R. Sapkota, K. K. Ojo, M. C. Roberts, Kellogg Schwab

Research output: Contribution to journalArticle

Abstract

Aims: In this study, multidrug-resistant bacteria previously recovered from the indoor air of a large-scale swine-feeding operation were tested for the presence of five macrolide, lincosamide and streptogramin (MLS) resistance genes and five tetracycline (tet) resistance genes. Methods and Results: Enterococcus spp. (n = 16) and Streptococcus spp. (n =16) were analysed using DNA-DNA hybridization, polymerase chain reaction (PCR) and oligoprobing of PCR products. All isolates carried multiple MLS resistance genes, while 50% of the Enterococcus spp. and 44% of the Streptococcus spp. also carried multiple tet resistance genes. All Enterococcus spp. carried erm(A) and erm(B), 69% carried erm(F), 44% carried mef(A), 75% carried tet(M), 69% carried tet(L) and 19% carried tet(K). All Streptococcus spp. carried erm(B), 94% carried erm(F), 75% carried erm(A), 38% carried mef(A), 50% carried tet(M), 81% carried tet(L) and 13% carried tet(K). Conclusions: Multidrug resistance among airborne bacteria recovered from a swine operation is encoded by multiple MLS and tet resistance genes. These are the first data regarding resistance gene carriage among airborne bacteria from swine-feeding operations. Significance and Impact of the Study: The high prevalence of multiple resistance genes reported here suggests that airborne Gram-positive bacteria from swine operations may be important contributors to environmental reservoirs of resistance genes.

Original languageEnglish (US)
Pages (from-to)534-540
Number of pages7
JournalLetters in Applied Microbiology
Volume43
Issue number5
DOIs
StatePublished - Nov 2006

Fingerprint

antibiotic resistance
Enterococcus
Antibiotics
indoor air
Microbial Drug Resistance
Tetracycline
Streptococcus
Swine
Genes
Air
Anti-Bacterial Agents
Streptogramins
gene
Lincosamides
Tetracycline Resistance
Bacteria
Macrolides
bacterium
Polymerase chain reaction
polymerase chain reaction

Keywords

  • Airborne bacteria
  • Antibiotic resistance genes
  • Enterococcus
  • Multidrug resistance
  • Streptococcus
  • Swine

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Water Science and Technology
  • Fluid Flow and Transfer Processes
  • Microbiology
  • Ecology

Cite this

Antibiotic resistance genes in multidrug-resistant Enterococcus spp. and Streptococcus spp. recovered from the indoor air of a large-scale swine-feeding operation. / Sapkota, A. R.; Ojo, K. K.; Roberts, M. C.; Schwab, Kellogg.

In: Letters in Applied Microbiology, Vol. 43, No. 5, 11.2006, p. 534-540.

Research output: Contribution to journalArticle

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abstract = "Aims: In this study, multidrug-resistant bacteria previously recovered from the indoor air of a large-scale swine-feeding operation were tested for the presence of five macrolide, lincosamide and streptogramin (MLS) resistance genes and five tetracycline (tet) resistance genes. Methods and Results: Enterococcus spp. (n = 16) and Streptococcus spp. (n =16) were analysed using DNA-DNA hybridization, polymerase chain reaction (PCR) and oligoprobing of PCR products. All isolates carried multiple MLS resistance genes, while 50{\%} of the Enterococcus spp. and 44{\%} of the Streptococcus spp. also carried multiple tet resistance genes. All Enterococcus spp. carried erm(A) and erm(B), 69{\%} carried erm(F), 44{\%} carried mef(A), 75{\%} carried tet(M), 69{\%} carried tet(L) and 19{\%} carried tet(K). All Streptococcus spp. carried erm(B), 94{\%} carried erm(F), 75{\%} carried erm(A), 38{\%} carried mef(A), 50{\%} carried tet(M), 81{\%} carried tet(L) and 13{\%} carried tet(K). Conclusions: Multidrug resistance among airborne bacteria recovered from a swine operation is encoded by multiple MLS and tet resistance genes. These are the first data regarding resistance gene carriage among airborne bacteria from swine-feeding operations. Significance and Impact of the Study: The high prevalence of multiple resistance genes reported here suggests that airborne Gram-positive bacteria from swine operations may be important contributors to environmental reservoirs of resistance genes.",
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