Antiallergic effect of anti-Siglec-F through reduction of eosinophilic inflammation in murine allergic rhinitis

Young Hyo Kim, Chang Shin Park, Dae Hyun Lim, Byong Kwan Son, Jeong Hee Kim, Sung Hye Ahn, Bruce S. Bochner, Kwangmin Na, Tae Young Jang

Research output: Contribution to journalArticle

Abstract

Background: Sialic acid-binding Ig-like lectin-F (Siglec-F) in mice and its functional paralog Siglec-8 in humans are transmembrane receptors that play a role in the apoptosis of eosinophils. We aimed to evaluate the therapeutic potential of anti-Siglec-F antibodies in a murine model of allergic rhinitis. Methods: Twenty-eight BALB/c mice were used. In group A (control group, n = 7), mice were sensitized and challenged with saline. In group B (ovalbumin [OVA] challenge group, n = 7), OVA was used for i.p. sensitization and intranasal challenge. Mice in group C (control IgG group, n = 7) or those in group D (anti-Siglec-F group, n = 7) had been given rabbit control IgG or anti-Siglec-F antibody injections, respectively. We assessed the number of nose-scratching events; serum total/OVA-specific IgE; the number of eosinophils, neutrophils, and lymphocytes in bronchoalveolar lavage (BAL) fluid; histopathological changes in nasal cavity tissues; and the levels of IL-4, IL-5, and IL-13 in BAL fluid. Results: Mice in group D had significantly less nose scratching. Serum total and OVA-specific IgE were not significantly changed. The number of eosinophils in BAL fluid and in the lamina propria of the nasal cavity mucosa was significantly decreased with anti-Siglec-F antibody treatment. The levels of Th2 cytokines such as IL-4, IL-5, and IL-13 were also significantly decreased with anti-Siglec-F antibody treatment. Conclusion: Anti-Siglec-F antibody has beneficial effects in a mouse model of experimental allergic rhinitis.

Original languageEnglish (US)
Pages (from-to)187-191
Number of pages5
JournalAmerican Journal of Rhinology and Allergy
Volume27
Issue number3
DOIs
Publication statusPublished - May 2013

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ASJC Scopus subject areas

  • Otorhinolaryngology
  • Immunology and Allergy

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