TY - JOUR
T1 - Anti-pruritic and anti-inflammatory effects of oxymatrine in a mouse model of allergic contact dermatitis
AU - Xu, Xiaoyun
AU - Xiao, Wei
AU - Zhang, Zhe
AU - Pan, Jianhao
AU - Yan, Yixi
AU - Zhu, Tao
AU - Tang, Dan
AU - Ye, Kaihe
AU - Paranjpe, Manish
AU - Qu, Lintao
AU - Nie, Hong
N1 - Funding Information:
This work was supported by grants from the Chinese National Natural Science Foundation to H.N. (No. 81373993 and 81673634), Innovation and entrepreneurship training of Jinan University to H.N. (No. 201610559016), Climbing project of Guangzhou province to H.N. (No. PDJH2016B0062).
Funding Information:
This work was supported by grants from the Chinese National Natural Science Foundation to H.N. (No. 81373993 and 81673634 ), Innovation and entrepreneurship training of Jinan University to H.N. (No. 201610559016 ), Climbing project of Guangzhou province to H.N. (No. PDJH2016B0062 ).
Publisher Copyright:
© 2018
PY - 2018/8
Y1 - 2018/8
N2 - Background: Allergic contact dermatitis (ACD) is a highly prevalent inflammatory disease of the skin. As a result of the complex etiology in ACD, therapeutic compounds targeting refractory pruritus in ACD lack efficacy and lead to numerous side effects. Objective: In this study, we investigated the anti-pruritic effects of oxymatrine (OMT) and explored its mechanism of action in a mouse model of ACD. Method: 72 male SPF C57BL/6 mice were randomly divided into control group, ACD model group, dexamethasone positive control group (0.08 mg kg−1) and 3 OMT groups (80, 40, 20 mg kg−1). OMT was administrated by intraperitoneal injection 1 h before video recording on day 10, 24 h after 2nd challenge with SADBE. Cheek skin fold thickness was measured before treatment and after recording. H&E staining was used for pathological observation. RT-qPCR, Immunohistochemistry and LEGENDplexTM assay were used to detect cytokines levels. The population of Treg cells in peripheral blood were detected via flow cytometry. Results: OMT treatment significantly decreases the skin inflammation and scratching bouts. It rescues defects in epidermal keratinization and inflammatory cell infiltration in ACD mice. Administration of OMT significantly reduced levels of IFN-γ IL-13, IL-17A, TNF-α IL-22 and mRNA expression of TNF-α and IL-1β. Furthermore, it increased the percentage of Treg cells in peripheral blood of ACD mice. Conclusion: We have demonstrated that OMT exhibits anti-pruritic and anti-inflammatory effects in ACD mice by regulating inflammatory mediators. OMT might emerge as a potential drug for the treatment of pruritus and skin inflammation in the setting of ACD.
AB - Background: Allergic contact dermatitis (ACD) is a highly prevalent inflammatory disease of the skin. As a result of the complex etiology in ACD, therapeutic compounds targeting refractory pruritus in ACD lack efficacy and lead to numerous side effects. Objective: In this study, we investigated the anti-pruritic effects of oxymatrine (OMT) and explored its mechanism of action in a mouse model of ACD. Method: 72 male SPF C57BL/6 mice were randomly divided into control group, ACD model group, dexamethasone positive control group (0.08 mg kg−1) and 3 OMT groups (80, 40, 20 mg kg−1). OMT was administrated by intraperitoneal injection 1 h before video recording on day 10, 24 h after 2nd challenge with SADBE. Cheek skin fold thickness was measured before treatment and after recording. H&E staining was used for pathological observation. RT-qPCR, Immunohistochemistry and LEGENDplexTM assay were used to detect cytokines levels. The population of Treg cells in peripheral blood were detected via flow cytometry. Results: OMT treatment significantly decreases the skin inflammation and scratching bouts. It rescues defects in epidermal keratinization and inflammatory cell infiltration in ACD mice. Administration of OMT significantly reduced levels of IFN-γ IL-13, IL-17A, TNF-α IL-22 and mRNA expression of TNF-α and IL-1β. Furthermore, it increased the percentage of Treg cells in peripheral blood of ACD mice. Conclusion: We have demonstrated that OMT exhibits anti-pruritic and anti-inflammatory effects in ACD mice by regulating inflammatory mediators. OMT might emerge as a potential drug for the treatment of pruritus and skin inflammation in the setting of ACD.
KW - Allergic contact dermatitis
KW - Anti-inflammatory
KW - Anti-pruritus
KW - Oxymatrine
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U2 - 10.1016/j.jdermsci.2018.04.009
DO - 10.1016/j.jdermsci.2018.04.009
M3 - Article
C2 - 29903654
AN - SCOPUS:85048281055
SN - 0923-1811
VL - 91
SP - 134
EP - 141
JO - Journal of Dermatological Science
JF - Journal of Dermatological Science
IS - 2
ER -