Anti-My-28, an antigranulocyte mouse monoclonal antibody, binds to a sugar sequence in lacto-N-neotetraose

S. L. Spitalnik; J. F. Schwartz; J. L. Magnani; D. D. Roberts; C. I. Civin; V. Ginsburg

Anti-My-28, an antigranulocyte mouse monoclonal antibody, binds to a sugar sequence in lacto-N-neotetraose

S. L. Spitalnik, J. F. Schwartz, J. L. Magnani, D. D. Roberts, C. I. Civin, V. Ginsburg

Research output: Contribution to journal › Article › peer-review

Abstract

Anti-My-28 is an IgM κ monoclonal antibody produced by a hybridoma prepared from spleen cells of a mouse immunized with normal human granulocytes. By immunofluorescence it binds to human granulocytes but not to monocyes and lymphocytes. However, after treating cells with neuramindase, the antibody also binds to lymphocytes and monocytes and to many leukemic cell lines and patient leukemic blast cells. Anti-My-28 binds to several neutral glycolipids and desialylated gangliosides of leukocytes and erythrocytes as detected by radioimmunoassay and immunostaining of thin-layer chromatograms. It recognizes a sugar sequence in lacto-N-neotetraose, Ga1β1-4GlcNacβ1-3Galβ1-4Glc. This tetrasaccharide occurs in the glycolipids paragloboside and sialosylparagloboside, and its distal trisaccharide sequence is found in higher glycolipids and in glycoproteins.

Original language	English (US)
Pages (from-to)	319-326
Number of pages	8
Journal	Blood
Volume	66
Issue number	2
State	Published - 1985
Externally published	Yes

ASJC Scopus subject areas

Hematology

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title = "Anti-My-28, an antigranulocyte mouse monoclonal antibody, binds to a sugar sequence in lacto-N-neotetraose",

abstract = "Anti-My-28 is an IgM κ monoclonal antibody produced by a hybridoma prepared from spleen cells of a mouse immunized with normal human granulocytes. By immunofluorescence it binds to human granulocytes but not to monocyes and lymphocytes. However, after treating cells with neuramindase, the antibody also binds to lymphocytes and monocytes and to many leukemic cell lines and patient leukemic blast cells. Anti-My-28 binds to several neutral glycolipids and desialylated gangliosides of leukocytes and erythrocytes as detected by radioimmunoassay and immunostaining of thin-layer chromatograms. It recognizes a sugar sequence in lacto-N-neotetraose, Ga1β1-4GlcNacβ1-3Galβ1-4Glc. This tetrasaccharide occurs in the glycolipids paragloboside and sialosylparagloboside, and its distal trisaccharide sequence is found in higher glycolipids and in glycoproteins.",

author = "Spitalnik, {S. L.} and Schwartz, {J. F.} and Magnani, {J. L.} and Roberts, {D. D.} and Civin, {C. I.} and V. Ginsburg",

year = "1985",

language = "English (US)",

volume = "66",

pages = "319--326",

journal = "Blood",

issn = "0006-4971",

publisher = "American Society of Hematology",

number = "2",

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T1 - Anti-My-28, an antigranulocyte mouse monoclonal antibody, binds to a sugar sequence in lacto-N-neotetraose

AU - Spitalnik, S. L.

AU - Schwartz, J. F.

AU - Magnani, J. L.

AU - Roberts, D. D.

AU - Civin, C. I.

AU - Ginsburg, V.

PY - 1985

Y1 - 1985

N2 - Anti-My-28 is an IgM κ monoclonal antibody produced by a hybridoma prepared from spleen cells of a mouse immunized with normal human granulocytes. By immunofluorescence it binds to human granulocytes but not to monocyes and lymphocytes. However, after treating cells with neuramindase, the antibody also binds to lymphocytes and monocytes and to many leukemic cell lines and patient leukemic blast cells. Anti-My-28 binds to several neutral glycolipids and desialylated gangliosides of leukocytes and erythrocytes as detected by radioimmunoassay and immunostaining of thin-layer chromatograms. It recognizes a sugar sequence in lacto-N-neotetraose, Ga1β1-4GlcNacβ1-3Galβ1-4Glc. This tetrasaccharide occurs in the glycolipids paragloboside and sialosylparagloboside, and its distal trisaccharide sequence is found in higher glycolipids and in glycoproteins.

AB - Anti-My-28 is an IgM κ monoclonal antibody produced by a hybridoma prepared from spleen cells of a mouse immunized with normal human granulocytes. By immunofluorescence it binds to human granulocytes but not to monocyes and lymphocytes. However, after treating cells with neuramindase, the antibody also binds to lymphocytes and monocytes and to many leukemic cell lines and patient leukemic blast cells. Anti-My-28 binds to several neutral glycolipids and desialylated gangliosides of leukocytes and erythrocytes as detected by radioimmunoassay and immunostaining of thin-layer chromatograms. It recognizes a sugar sequence in lacto-N-neotetraose, Ga1β1-4GlcNacβ1-3Galβ1-4Glc. This tetrasaccharide occurs in the glycolipids paragloboside and sialosylparagloboside, and its distal trisaccharide sequence is found in higher glycolipids and in glycoproteins.

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