Anti-inflammatory effect of cyclosporin a on human skin mast cells

Cristiana Stellato, Amato de Paulis, Anna Ciccarelli, Raffaele Cirillo, Vincenzo Patella, Vincenzo Casolaro, Gianni Marone

Research output: Contribution to journalArticle

Abstract

We have examined the effects of cyclosporin A (CsA) and cyclosporin H (CsH), which bind with different affinity to cyclophilin, to evaluate the role of this protein in the release of preformed (histamine) and de novo synthesized (prostaglandin D2[PGD2]) mediators of inflammatory reactions from human skin mast cells (HSMC). CsA (2.4-800 nM)inhibited (5-60%) histamine release from HSMC challenged with anti-IgE. CsA exerted little, if any, inhibitory effect on histamine release from HSMC challenged with compound A23187 and substance P, whereas it completely suppressed A23187-induced histamine release from human basophils. Inhibition of histamine release from HSMC challenged with anti-IgE was extremely rapid and was not abolished by washing (three times) the cells before anti-IgE challenge. CsA (2.4-800 nM) markedly inhibited (25-70%) the de novo synthesis of PGD2 from HSMC challenged with anti-IgE. CsH, which has an extremely low affinity for cyclophilin, had no effect on skin mast-cell mediator release. These data suggest that CsA is a potent anti-inflammatory agent acting on HSMC, presumably by interacting with cyclophilin.

Original languageEnglish (US)
Pages (from-to)800-804
Number of pages5
JournalJournal of Investigative Dermatology
Volume98
Issue number5
StatePublished - May 1992
Externally publishedYes

ASJC Scopus subject areas

  • Dermatology

Fingerprint Dive into the research topics of 'Anti-inflammatory effect of cyclosporin a on human skin mast cells'. Together they form a unique fingerprint.

  • Cite this

    Stellato, C., de Paulis, A., Ciccarelli, A., Cirillo, R., Patella, V., Casolaro, V., & Marone, G. (1992). Anti-inflammatory effect of cyclosporin a on human skin mast cells. Journal of Investigative Dermatology, 98(5), 800-804.