TY - JOUR
T1 - Anti-inflammatory effect of allylpyrocatechol in LPS-induced macrophages is mediated by suppression of iNOS and COX-2 via the NF-κB pathway
AU - Sarkar, Debjani
AU - Saha, Piu
AU - Gamre, Sunita
AU - Bhattacharjee, Surajit
AU - Hariharan, Chellaram
AU - Ganguly, Sudipto
AU - Sen, Rupashree
AU - Mandal, Goutam
AU - Chattopadhyay, Subrata
AU - Majumdar, Subrata
AU - Chatterjee, Mitali
PY - 2008/9/1
Y1 - 2008/9/1
N2 - The crude ethanol extract of Piper betle leaf is reported to possess anti-inflammatory activity which has been suggested to be mediated by allylpyrocatechol (APC). In the present study, we have demonstrated the anti-inflammatory effects of APC (10 mg/kg, p.o.) in an animal model of inflammation. To investigate the mechanism(s) of this anti-inflammatory activity, we examined its effects on the lipopolysaccaride (LPS)-induced production of NO and PGE2 in a murine macrophage cell line, RAW 264.7. APC inhibited production of NO and PGE2 in a dose dependent manner as also decreased mRNA expression of iNOS, COX-2, IL-12p40 and TNF-alpha. Since nuclear factor-κB (NF-κB) appears to play a central role in transcriptional regulation of these proteins, we investigated the effects of APC on this transcription factor. APC inhibited LPS induced nuclear factor-kappaB (NF-κB) activation, by preventing degradation of the inhibitor kappaB (IκB). Taken together, our data indicates that APC targets the inflammatory response of macrophages via inhibition of iNOS, COX-2 and IL-12 p40 through down regulation of the NF-κB pathway, indicating that APC may have therapeutic potential in inflammation associated disorders.
AB - The crude ethanol extract of Piper betle leaf is reported to possess anti-inflammatory activity which has been suggested to be mediated by allylpyrocatechol (APC). In the present study, we have demonstrated the anti-inflammatory effects of APC (10 mg/kg, p.o.) in an animal model of inflammation. To investigate the mechanism(s) of this anti-inflammatory activity, we examined its effects on the lipopolysaccaride (LPS)-induced production of NO and PGE2 in a murine macrophage cell line, RAW 264.7. APC inhibited production of NO and PGE2 in a dose dependent manner as also decreased mRNA expression of iNOS, COX-2, IL-12p40 and TNF-alpha. Since nuclear factor-κB (NF-κB) appears to play a central role in transcriptional regulation of these proteins, we investigated the effects of APC on this transcription factor. APC inhibited LPS induced nuclear factor-kappaB (NF-κB) activation, by preventing degradation of the inhibitor kappaB (IκB). Taken together, our data indicates that APC targets the inflammatory response of macrophages via inhibition of iNOS, COX-2 and IL-12 p40 through down regulation of the NF-κB pathway, indicating that APC may have therapeutic potential in inflammation associated disorders.
KW - Allylpyrocatechol
KW - Anti-inflammatory
KW - Nitric oxide
KW - Nuclear factor-κB
KW - Piper betle
UR - http://www.scopus.com/inward/record.url?scp=46349093408&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=46349093408&partnerID=8YFLogxK
U2 - 10.1016/j.intimp.2008.05.003
DO - 10.1016/j.intimp.2008.05.003
M3 - Article
C2 - 18602073
AN - SCOPUS:46349093408
VL - 8
SP - 1264
EP - 1271
JO - International Immunopharmacology
JF - International Immunopharmacology
SN - 1567-5769
IS - 9
ER -