TY - JOUR
T1 - Anti-inflammatory effect of allylpyrocatechol in LPS-induced macrophages is mediated by suppression of iNOS and COX-2 via the NF-κB pathway
AU - Sarkar, Debjani
AU - Saha, Piu
AU - Gamre, Sunita
AU - Bhattacharjee, Surajit
AU - Hariharan, Chellaram
AU - Ganguly, Sudipto
AU - Sen, Rupashree
AU - Mandal, Goutam
AU - Chattopadhyay, Subrata
AU - Majumdar, Subrata
AU - Chatterjee, Mitali
N1 - Funding Information:
This work was supported by Life Sciences Research Board, DRDO, Govt. of India and Dept. of Science & Technology, Govt. of West Bengal, India. RS and PS are recipients of Senior Research Fellowships from Indian Council of Medical Research, SG and GM are recipients of Senior Research Fellowships from University Grants Commission and Council for Scientific and Industrial Research, Govt. of India respectively.
PY - 2008/9
Y1 - 2008/9
N2 - The crude ethanol extract of Piper betle leaf is reported to possess anti-inflammatory activity which has been suggested to be mediated by allylpyrocatechol (APC). In the present study, we have demonstrated the anti-inflammatory effects of APC (10 mg/kg, p.o.) in an animal model of inflammation. To investigate the mechanism(s) of this anti-inflammatory activity, we examined its effects on the lipopolysaccaride (LPS)-induced production of NO and PGE2 in a murine macrophage cell line, RAW 264.7. APC inhibited production of NO and PGE2 in a dose dependent manner as also decreased mRNA expression of iNOS, COX-2, IL-12p40 and TNF-alpha. Since nuclear factor-κB (NF-κB) appears to play a central role in transcriptional regulation of these proteins, we investigated the effects of APC on this transcription factor. APC inhibited LPS induced nuclear factor-kappaB (NF-κB) activation, by preventing degradation of the inhibitor kappaB (IκB). Taken together, our data indicates that APC targets the inflammatory response of macrophages via inhibition of iNOS, COX-2 and IL-12 p40 through down regulation of the NF-κB pathway, indicating that APC may have therapeutic potential in inflammation associated disorders.
AB - The crude ethanol extract of Piper betle leaf is reported to possess anti-inflammatory activity which has been suggested to be mediated by allylpyrocatechol (APC). In the present study, we have demonstrated the anti-inflammatory effects of APC (10 mg/kg, p.o.) in an animal model of inflammation. To investigate the mechanism(s) of this anti-inflammatory activity, we examined its effects on the lipopolysaccaride (LPS)-induced production of NO and PGE2 in a murine macrophage cell line, RAW 264.7. APC inhibited production of NO and PGE2 in a dose dependent manner as also decreased mRNA expression of iNOS, COX-2, IL-12p40 and TNF-alpha. Since nuclear factor-κB (NF-κB) appears to play a central role in transcriptional regulation of these proteins, we investigated the effects of APC on this transcription factor. APC inhibited LPS induced nuclear factor-kappaB (NF-κB) activation, by preventing degradation of the inhibitor kappaB (IκB). Taken together, our data indicates that APC targets the inflammatory response of macrophages via inhibition of iNOS, COX-2 and IL-12 p40 through down regulation of the NF-κB pathway, indicating that APC may have therapeutic potential in inflammation associated disorders.
KW - Allylpyrocatechol
KW - Anti-inflammatory
KW - Nitric oxide
KW - Nuclear factor-κB
KW - Piper betle
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U2 - 10.1016/j.intimp.2008.05.003
DO - 10.1016/j.intimp.2008.05.003
M3 - Article
C2 - 18602073
AN - SCOPUS:46349093408
SN - 1567-5769
VL - 8
SP - 1264
EP - 1271
JO - International immunopharmacology
JF - International immunopharmacology
IS - 9
ER -