Anti-HLA class I antibody inhibits Staphylococcus enterotoxin A (SEA)-induced proliferation of human PBMC

Padmavathi Baskar, James E K Hildreth, Francis J. Chrest, James E. Nagel, William H. Adler

    Research output: Contribution to journalArticle

    Abstract

    The antigen-presenting role of major histocompatibility complex (MHC) Class I molecules in the activation of appropriately restricted T cells is well documented. Now growing evidence indicates that MHC Class I molecules can, in addition, exert a regulatory effect and influence the resulting immune responses. In this report, we show that a monoclonal antibody (mAb) directed against a conserved region of the human leukocyte antigens (HLA)-A, -B, and -C was able to inhibit proliferation of human peripheral blood mononuclear cells induced by the superantigen, Staphylococcus enterotoxin A. While anti-HLA inhibition was associated with a decrease in IL-2 receptor (IL-2R) expression, the addition of exogenous IL-2 did not restore the proliferative response in the presence of anti-HLA mAb. The inhibition of DNA synthesis was also associated with a decrease in the expression of the early activation marker CD69. These results suggest a critical role for HLA Class I molecules in the early events of human lymphocyte activation and proliferation as well as in their expression of the IL-2R.

    Original languageEnglish (US)
    Pages (from-to)135-138
    Number of pages4
    JournalCellular Immunology
    Volume172
    Issue number1
    DOIs
    StatePublished - Aug 25 1996

    ASJC Scopus subject areas

    • Cell Biology
    • Immunology

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