TY - JOUR
T1 - Anti-histone antibodies in the serum of autoimmune MRL and NZB NZW1 F1 mice
AU - Brick, James E.
AU - Ong, Shu Hui
AU - Bathon, Joan M.
AU - Walker, Sara E.
AU - O'Sullivan, Frank X.
AU - DiBartolomeo, Anthony G.
PY - 1990
Y1 - 1990
N2 - Anti-histone antibodies have been reported in a number of human autoimmune diseases, most notably idiopathic and drug-induced lupus erythematosus. In the current study, anti-histone antibody activity was detected using ELISA and electroblotting techniques in sera from autoimmune NZB W, MRL-lpr, and MRL-+/+ mice. Anti-histone activity increased with age, maturing earlier in females, in both NZB W and MRL-lpr mice. Testosterone treatment decreased anti-histone activity in NZB W mice and estrogen treatment from 2 weeks of age increased anti-histone activity in MRL-lpr mice, suggesting that gonadal hormones modified the expression of autoantibodies recognizing these protein antigens. Estrogen also increased serum IgG levels in MRL-lpr mice. Sex hormones affected expression of antibodies recognizing soy milk proteins but not ovalbumin in a similar manner. Nitrocellulose Western blots of SDS gels probed with sera from both types of autoimmune mice most often demonstrated reactivity with histone1. Some mice, usually mature females, also recognized histone4, histone3, and histone2.
AB - Anti-histone antibodies have been reported in a number of human autoimmune diseases, most notably idiopathic and drug-induced lupus erythematosus. In the current study, anti-histone antibody activity was detected using ELISA and electroblotting techniques in sera from autoimmune NZB W, MRL-lpr, and MRL-+/+ mice. Anti-histone activity increased with age, maturing earlier in females, in both NZB W and MRL-lpr mice. Testosterone treatment decreased anti-histone activity in NZB W mice and estrogen treatment from 2 weeks of age increased anti-histone activity in MRL-lpr mice, suggesting that gonadal hormones modified the expression of autoantibodies recognizing these protein antigens. Estrogen also increased serum IgG levels in MRL-lpr mice. Sex hormones affected expression of antibodies recognizing soy milk proteins but not ovalbumin in a similar manner. Nitrocellulose Western blots of SDS gels probed with sera from both types of autoimmune mice most often demonstrated reactivity with histone1. Some mice, usually mature females, also recognized histone4, histone3, and histone2.
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U2 - 10.1016/0090-1229(90)90051-Q
DO - 10.1016/0090-1229(90)90051-Q
M3 - Article
C2 - 2302840
AN - SCOPUS:0025275479
SN - 0090-1229
VL - 54
SP - 372
EP - 381
JO - Clinical Immunology and Immunopathology
JF - Clinical Immunology and Immunopathology
IS - 3
ER -