Anti-hepatitis C virus activity of albinterferon alfa-2b in cell culture

Chen Liu, Haizhen Zhu, Mani G. Subramanian, Paul A. Moore, Yiling Xu, David R. Nelson

Research output: Contribution to journalArticlepeer-review

Abstract

Background: Interferon-based combination therapy is the standard treatment for chronic hepatitis C virus (HCV) infection. The weekly administration of long-acting pegylated interferons (PEG-IFNα-2a or PEG-IFNα-2b) provides superior antiviral efficacy over standard interferon alfa (IFNα) for the treatment of HCV infection. Albinterferon alfa-2b (alb-IFN) is a novel recombinant protein consisting of IFNα-2b that is genetically fused to human albumin. Methods: To test alb-IFN antiviral efficacy, we compared the antiviral activity of unmodified IFNα with the three modified interferons (PEG-IFNα-2a, PEG-IFNα-2b, and alb-IFN) at clinically relevant serum concentrations using liver cell-based and non-liver cell-based HCV replicon cell lines. The EC 50 in GSB cells for IFNα-2b, PEG-IFNα-2a, PEG-IFNα-2b and alb-IFN was 7 U/mL, 1.1 ng/mL, 18 ng/mL, and 15 ng/mL, respectively. Results: At clinically relevant patient serum concentrations, alb-IFN exhibits more antiviral activity than the pegylated interferons. Alb-IFN showed similar inhibition of HCV replication in human liver cells and non-liver cells, indicating it has anti-HCV activity in non-liver cells. The magnitude of induction of interferon-stimulated genes (MxA, 2′5′ OAS1, IFI44, and IFI27) at 6 h and 48 h was comparable for all the modified IFNs in human liver cells at the drug concentrations evaluated. Conclusion: The present study indicates that alb-IFN has a potent, direct anti-HCV activity in both liver and non-liver cells.

Original languageEnglish (US)
Pages (from-to)941-947
Number of pages7
JournalHepatology Research
Volume37
Issue number11
DOIs
StatePublished - Nov 1 2007

Keywords

  • Albinterferon alfa-2b
  • Gene expression
  • Hepatitis C virus
  • Replicon

ASJC Scopus subject areas

  • Hepatology
  • Infectious Diseases

Fingerprint Dive into the research topics of 'Anti-hepatitis C virus activity of albinterferon alfa-2b in cell culture'. Together they form a unique fingerprint.

Cite this