Anti-ganglioside antibodies bind with enhanced affinity to gangliosides containing very long chain fatty acids

Y. Tagawa, W. Laroy, L. Nimrichter, S. E. Fromholt, Ann B. Moser, H. W. Moser, Ronald Lee Schnaar

Research output: Contribution to journalArticle

Abstract

Gangliosides function in both physiological and pathological molecular recognition. Although much research has focused on the role of ganglioside glycans in recognition, fewer studies have addressed the role of the ceramide moiety. Ceramides of major brain gangliosides are composed predominantly of monounsaturated 18-carbon and 20-carbon long chain bases with a saturated 18-carbon fatty acid amide. In contrast, gangliosides of X-linked adrenoleukodystrophy patients are characterized by abnormal very long chain fatty acids that are proposed to be associated with autoimmune inflammation. In the current study we synthesized and characterized derivatives of the major brain ganglioside GD1a bearing defined very long chain fatty acid amides (C24:0, C24:1, and C26:0). When tested in a solid phase binding assay in the presence of auxiliary membrane lipids, GD1a species with long chain fatty acids were up to 8-fold more potent than normal brain GD1a in binding four different anti-GD1a monoclonal antibodies. These data support the hypothesis that gangliosides bearing very long chain fatty acids are differentially displayed on membranes, which may lead to altered antigenicity.

Original languageEnglish (US)
Pages (from-to)847-855
Number of pages9
JournalNeurochemical Research
Volume27
Issue number7-8
DOIs
StatePublished - Aug 2002

Fingerprint

Gangliosides
Anti-Idiotypic Antibodies
Fatty Acids
Bearings (structural)
Antibodies
Brain
Carbon
Ceramides
Amides
Adrenoleukodystrophy
Molecular recognition
Membrane Lipids
Polysaccharides
Assays
Monoclonal Antibodies
Inflammation
Derivatives
Membranes
Research

Keywords

  • Adrenoleukodystrophy
  • Autoimmune disease
  • Ceramide
  • GD1a

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry

Cite this

Anti-ganglioside antibodies bind with enhanced affinity to gangliosides containing very long chain fatty acids. / Tagawa, Y.; Laroy, W.; Nimrichter, L.; Fromholt, S. E.; Moser, Ann B.; Moser, H. W.; Schnaar, Ronald Lee.

In: Neurochemical Research, Vol. 27, No. 7-8, 08.2002, p. 847-855.

Research output: Contribution to journalArticle

Tagawa, Y. ; Laroy, W. ; Nimrichter, L. ; Fromholt, S. E. ; Moser, Ann B. ; Moser, H. W. ; Schnaar, Ronald Lee. / Anti-ganglioside antibodies bind with enhanced affinity to gangliosides containing very long chain fatty acids. In: Neurochemical Research. 2002 ; Vol. 27, No. 7-8. pp. 847-855.
@article{92f3c92e36f74a999416b1acca355167,
title = "Anti-ganglioside antibodies bind with enhanced affinity to gangliosides containing very long chain fatty acids",
abstract = "Gangliosides function in both physiological and pathological molecular recognition. Although much research has focused on the role of ganglioside glycans in recognition, fewer studies have addressed the role of the ceramide moiety. Ceramides of major brain gangliosides are composed predominantly of monounsaturated 18-carbon and 20-carbon long chain bases with a saturated 18-carbon fatty acid amide. In contrast, gangliosides of X-linked adrenoleukodystrophy patients are characterized by abnormal very long chain fatty acids that are proposed to be associated with autoimmune inflammation. In the current study we synthesized and characterized derivatives of the major brain ganglioside GD1a bearing defined very long chain fatty acid amides (C24:0, C24:1, and C26:0). When tested in a solid phase binding assay in the presence of auxiliary membrane lipids, GD1a species with long chain fatty acids were up to 8-fold more potent than normal brain GD1a in binding four different anti-GD1a monoclonal antibodies. These data support the hypothesis that gangliosides bearing very long chain fatty acids are differentially displayed on membranes, which may lead to altered antigenicity.",
keywords = "Adrenoleukodystrophy, Autoimmune disease, Ceramide, GD1a",
author = "Y. Tagawa and W. Laroy and L. Nimrichter and Fromholt, {S. E.} and Moser, {Ann B.} and Moser, {H. W.} and Schnaar, {Ronald Lee}",
year = "2002",
month = "8",
doi = "10.1023/A:1020221410895",
language = "English (US)",
volume = "27",
pages = "847--855",
journal = "Neurochemical Research",
issn = "0364-3190",
publisher = "Springer New York",
number = "7-8",

}

TY - JOUR

T1 - Anti-ganglioside antibodies bind with enhanced affinity to gangliosides containing very long chain fatty acids

AU - Tagawa, Y.

AU - Laroy, W.

AU - Nimrichter, L.

AU - Fromholt, S. E.

AU - Moser, Ann B.

AU - Moser, H. W.

AU - Schnaar, Ronald Lee

PY - 2002/8

Y1 - 2002/8

N2 - Gangliosides function in both physiological and pathological molecular recognition. Although much research has focused on the role of ganglioside glycans in recognition, fewer studies have addressed the role of the ceramide moiety. Ceramides of major brain gangliosides are composed predominantly of monounsaturated 18-carbon and 20-carbon long chain bases with a saturated 18-carbon fatty acid amide. In contrast, gangliosides of X-linked adrenoleukodystrophy patients are characterized by abnormal very long chain fatty acids that are proposed to be associated with autoimmune inflammation. In the current study we synthesized and characterized derivatives of the major brain ganglioside GD1a bearing defined very long chain fatty acid amides (C24:0, C24:1, and C26:0). When tested in a solid phase binding assay in the presence of auxiliary membrane lipids, GD1a species with long chain fatty acids were up to 8-fold more potent than normal brain GD1a in binding four different anti-GD1a monoclonal antibodies. These data support the hypothesis that gangliosides bearing very long chain fatty acids are differentially displayed on membranes, which may lead to altered antigenicity.

AB - Gangliosides function in both physiological and pathological molecular recognition. Although much research has focused on the role of ganglioside glycans in recognition, fewer studies have addressed the role of the ceramide moiety. Ceramides of major brain gangliosides are composed predominantly of monounsaturated 18-carbon and 20-carbon long chain bases with a saturated 18-carbon fatty acid amide. In contrast, gangliosides of X-linked adrenoleukodystrophy patients are characterized by abnormal very long chain fatty acids that are proposed to be associated with autoimmune inflammation. In the current study we synthesized and characterized derivatives of the major brain ganglioside GD1a bearing defined very long chain fatty acid amides (C24:0, C24:1, and C26:0). When tested in a solid phase binding assay in the presence of auxiliary membrane lipids, GD1a species with long chain fatty acids were up to 8-fold more potent than normal brain GD1a in binding four different anti-GD1a monoclonal antibodies. These data support the hypothesis that gangliosides bearing very long chain fatty acids are differentially displayed on membranes, which may lead to altered antigenicity.

KW - Adrenoleukodystrophy

KW - Autoimmune disease

KW - Ceramide

KW - GD1a

UR - http://www.scopus.com/inward/record.url?scp=0036694863&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036694863&partnerID=8YFLogxK

U2 - 10.1023/A:1020221410895

DO - 10.1023/A:1020221410895

M3 - Article

C2 - 12374222

AN - SCOPUS:0036694863

VL - 27

SP - 847

EP - 855

JO - Neurochemical Research

JF - Neurochemical Research

SN - 0364-3190

IS - 7-8

ER -