Anti-epidermal-cell-surface pemphigus antibody detaches viable epidermal cells from culture plates by activation of proteinase

R. M. Farb, R. Dykes, G. S. Lazarus

Research output: Contribution to journalArticle

Abstract

Immunoglobulin from pemphigus patients binds to the surface of mouse epidermal cells in culture. Cells incubated with the pemphigus antibody are easily detached from culture plates whereas cells incubated with serum from normal patients remain on the plate. Pemphigus antibody-mediated cell detachment is blocked by the addition of the proteinase inhibitors soybean trypsin inhibitor and α2-macroglobulin to the culture media. Detachable cells are viable, and activation of the complement cascade is not necessary for cell detachment. The anti-cell-surface antibody of pemphigus appears to disrupt adhesion between viable epidermal cells by activation of proteinase.

Original languageEnglish (US)
Pages (from-to)459-463
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume75
Issue number1
DOIs
StatePublished - Jan 1 1978
Externally publishedYes

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