Anti-complement immunofluorescence establishes nuclear localization of human cytomegalovirus matrix protein

Diana Weiner, D Wade Gibson, Kay L. Fields

Research output: Contribution to journalArticle

Abstract

A monospecific, polyclonal antiserum to the 69-kDa matrix protein of human cytomegalovirus (HCMV) was prepared in a guinea pig and used to determine the intracellular distribution of this viral antigen. The resulting antiserum was specific for infected cells as tested by immunofluorescence, and specific for the HCMV matrix protein as determined by "nitrocellulose immunoassay" of electrophoretically separated, infected-cell proteins. Antibodies were reacted with fixed, infected human fibroblasts, and visualized by the anticomplement immunofluorescence procedure to avoid complications arising from the strong IgG Fc binding activity of the infected-cell-specific cytoplasmic inclusion. Results establish that the matrix protein is located in the nucleus, and indicate that it is concentrated in the nucleoplasm rather than within the intranuclear inclusions.

Original languageEnglish (US)
Pages (from-to)19-28
Number of pages10
JournalVirology
Volume147
Issue number1
DOIs
StatePublished - 1985

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Cytomegalovirus
Fluorescent Antibody Technique
Immune Sera
Proteins
Intranuclear Inclusion Bodies
Collodion
Viral Antigens
Inclusion Bodies
Immunoassay
Guinea Pigs
Fibroblasts
Immunoglobulin G
Antibodies

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Anti-complement immunofluorescence establishes nuclear localization of human cytomegalovirus matrix protein. / Weiner, Diana; Gibson, D Wade; Fields, Kay L.

In: Virology, Vol. 147, No. 1, 1985, p. 19-28.

Research output: Contribution to journalArticle

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