TY - JOUR
T1 - Anti-complement immunofluorescence establishes nuclear localization of human cytomegalovirus matrix protein
AU - Weiner, Diana
AU - Gibson, Wade
AU - Fields, Kay L.
N1 - Funding Information:
We thank Diane Hayward for directing our attention to the advantages of the ACIF technique. This work was aided by NIH Grants NS 14580, AI 13718, Al 16959, and AI 193’73. Diana Weiner was a predor-toral fellow of the Biochemistry, Cellular and Molecular Biology Training Program at the Johns Hopkins University School of Medicine during the course of this work.
PY - 1985/11
Y1 - 1985/11
N2 - A monospecific, polyclonal antiserum to the 69-kDa matrix protein of human cytomegalovirus (HCMV) was prepared in a guinea pig and used to determine the intracellular distribution of this viral antigen. The resulting antiserum was specific for infected cells as tested by immunofluorescence, and specific for the HCMV matrix protein as determined by "nitrocellulose immunoassay" of electrophoretically separated, infected-cell proteins. Antibodies were reacted with fixed, infected human fibroblasts, and visualized by the anticomplement immunofluorescence procedure to avoid complications arising from the strong IgG Fc binding activity of the infected-cell-specific cytoplasmic inclusion. Results establish that the matrix protein is located in the nucleus, and indicate that it is concentrated in the nucleoplasm rather than within the intranuclear inclusions.
AB - A monospecific, polyclonal antiserum to the 69-kDa matrix protein of human cytomegalovirus (HCMV) was prepared in a guinea pig and used to determine the intracellular distribution of this viral antigen. The resulting antiserum was specific for infected cells as tested by immunofluorescence, and specific for the HCMV matrix protein as determined by "nitrocellulose immunoassay" of electrophoretically separated, infected-cell proteins. Antibodies were reacted with fixed, infected human fibroblasts, and visualized by the anticomplement immunofluorescence procedure to avoid complications arising from the strong IgG Fc binding activity of the infected-cell-specific cytoplasmic inclusion. Results establish that the matrix protein is located in the nucleus, and indicate that it is concentrated in the nucleoplasm rather than within the intranuclear inclusions.
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U2 - 10.1016/0042-6822(85)90223-5
DO - 10.1016/0042-6822(85)90223-5
M3 - Article
C2 - 2998062
AN - SCOPUS:0022404567
SN - 0042-6822
VL - 147
SP - 19
EP - 28
JO - Virology
JF - Virology
IS - 1
ER -