Anti-basal ganglia antibody abnormalities in Sydenham chorea

Harvey S. Singer, Christopher R. Loiselle, Olivia Lee, Marjorie A. Garvey, Franz H. Grus

Research output: Contribution to journalArticle

Abstract

Anti-basal ganglia antibodies (ABGA) were measured in nine children with Sydenham chorea (SC) and compared to nine controls. Enzyme-linked immunosorbent assay (ELISA) and Western blot (WB) methods were used to detect ABGA against supernatant (S1), pellet, and synaptosomal preparations from adult and pediatric postmortem caudate, putamen, and globus pallidus. ELISA optical density (OD) values were higher in SC patients than controls across all preparations, but did not reach a level of significance. Although WB identified multiple bands in all subjects, discriminant analysis showed that the mean binding patterns of SC patients were significantly different from control, most notably in the caudate S1 fraction (Wilks' λ=0.011, p<0.0001). Numerous antigens contributed to differences between groups; the two most defining molecular masses were at 126 and 113 kDa. In contrast to WB with discriminant analysis, ELISA measurements did not significantly differentiate between the SC group and controls.

Original languageEnglish (US)
Pages (from-to)154-161
Number of pages8
JournalJournal of Neuroimmunology
Volume136
Issue number1-2
DOIs
StatePublished - Mar 2003

Keywords

  • Anti-basal ganglia antibodies
  • Discriminant analysis
  • ELISA
  • Sydenham chorea
  • Western blot

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Neurology
  • Clinical Neurology

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