Anti-basal ganglia antibodies in PANDAS

Harvey Singer, Christopher R. Loiselle, Olivia Lee, Karen Minzer, Susan Swedo, Franz H. Grus

Research output: Contribution to journalArticle

Abstract

An autoimmune-mediated mechanism involving molecular mimicry has been proposed for a variety of pediatric movement disorders that occur after a streptococcal infection. In this study, anti-basal ganglia antibodies (ABGA) were measured in 15 children with the diagnosis of pediatric autoimmune neuropsychiatric disorder associated with streptococcal infection (PANDAS) and compared with those in 15 controls. ELISA and Western immunoblotting (WB) methods were used to detect ABGA against supernatant (S1), pellet (P2), and synaptosomal preparations from adult postmortem caudate, putamen, and globus pallidus. ELISA optical density values did not differ between PANDAS patients and controls across all preparations. Immunoblotting identified multiple bands in all subjects with no differences in the number of bands or their total density. Discriminant analysis, used to assess mean binding patterns, showed that PANDAS patients differed from controls only for the caudate S1 fraction (Wilks' λ = 0.0236, P <0.0002), with PANDAS-primarily tic subjects providing the greatest discrimination. Among the epitopes contributing to differences between PANDAS and control in the caudate S1 fraction, mean binding to the epitope at 183 kDa was the most different between groups. In conclusion, ELISA measurements do not differentiate between PANDAS and controls, suggesting a lack of major antibody changes in this disorder. Further immunoblot analyses using a caudate supernatant fraction are required to completely exclude the possibility of minor antibody repertoire differences in PANDAS subjects, especially in those who primarily have tics.

Original languageEnglish (US)
Pages (from-to)406-415
Number of pages10
JournalMovement disorders : official journal of the Movement Disorder Society
Volume19
Issue number4
DOIs
StatePublished - Apr 2004

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Basal Ganglia
Antibodies
Infection Control
Tics
Enzyme-Linked Immunosorbent Assay
Epitopes
Molecular Mimicry
Streptococcal Infections
Globus Pallidus
Putamen
Movement Disorders
Discriminant Analysis
Pediatric Autoimmune Neuropsychiatric Disorders Associated with Streptococcal infections
Immunoblotting
Western Blotting
Pediatrics

Keywords

  • Anti-basal ganglia antibodies
  • Discriminant analysis
  • ELISA
  • PANDAS
  • Western blot

ASJC Scopus subject areas

  • Clinical Neurology
  • Neuroscience(all)

Cite this

Anti-basal ganglia antibodies in PANDAS. / Singer, Harvey; Loiselle, Christopher R.; Lee, Olivia; Minzer, Karen; Swedo, Susan; Grus, Franz H.

In: Movement disorders : official journal of the Movement Disorder Society, Vol. 19, No. 4, 04.2004, p. 406-415.

Research output: Contribution to journalArticle

Singer, Harvey ; Loiselle, Christopher R. ; Lee, Olivia ; Minzer, Karen ; Swedo, Susan ; Grus, Franz H. / Anti-basal ganglia antibodies in PANDAS. In: Movement disorders : official journal of the Movement Disorder Society. 2004 ; Vol. 19, No. 4. pp. 406-415.
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