Anti-angiogenic activity of torilin, a sesquiterpene compound isolated from Torilis japonica

Myoung Sook Kim, You Mie Lee, Eun Joung Moon, Se Eun Kim, Jung Joon Lee, Kyu Won Kim

Research output: Contribution to journalArticle

Abstract

Torilin is a sesquiterpene compound purified from fruits of Torilis japonica (Umbelliferae). In this study, we demonstrated the anti-angiogenic activity of torilin using in vivo and in vitro assay systems. Torilin decreased both neovascularization of chick embryos in the chorioallantoic membrane assay and basic fibroblast growth factor-induced vessel formation in the mouse Matrigel plug assay. Torilin also reduced the proliferation and tube formation of human umbilical vein endothelial cells. In addition, the concentrated conditioned media obtained from torilin-treated HepG2 human hepatoblastoma cells blocked the angiogenic activation of torilin-untreated concentrated conditioned media, indicating that torilin may have an inhibitory effect on tumor-induced angiogenesis. To determine what molecules were involved in the anti-angiogenic activity, we examined the expression of hypoxia-inducible angiogenic factors in torilin-treated HepG2 cells. Torilin significantly down-regulated the expression of hypoxia-inducible vascular endothelial growth factor and insulin-like growth factor-11. Taken together, our data suggest that torilin may be a strong angiogenic inhibitor with the ability to decrease tube formation of vascular endothelial cells and to reduce expression of angiogenic factors of tumor cells. (C) 2000 Wiley-Liss, Inc.

Original languageEnglish (US)
Pages (from-to)269-275
Number of pages7
JournalInternational Journal of Cancer
Volume87
Issue number2
DOIs
StatePublished - Jul 15 2000
Externally publishedYes

Fingerprint

Sesquiterpenes
Angiogenesis Inducing Agents
Conditioned Culture Medium
torilin
Apiaceae
Hepatoblastoma
Chorioallantoic Membrane
Angiogenesis Inhibitors
Human Umbilical Vein Endothelial Cells
Hep G2 Cells
Somatomedins
Fibroblast Growth Factor 2
Chick Embryo
Vascular Endothelial Growth Factor A
Fruit
Endothelial Cells

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Anti-angiogenic activity of torilin, a sesquiterpene compound isolated from Torilis japonica. / Kim, Myoung Sook; Lee, You Mie; Moon, Eun Joung; Kim, Se Eun; Lee, Jung Joon; Kim, Kyu Won.

In: International Journal of Cancer, Vol. 87, No. 2, 15.07.2000, p. 269-275.

Research output: Contribution to journalArticle

Kim, Myoung Sook ; Lee, You Mie ; Moon, Eun Joung ; Kim, Se Eun ; Lee, Jung Joon ; Kim, Kyu Won. / Anti-angiogenic activity of torilin, a sesquiterpene compound isolated from Torilis japonica. In: International Journal of Cancer. 2000 ; Vol. 87, No. 2. pp. 269-275.
@article{22c70ffa845c44d7b21a63f06b23957b,
title = "Anti-angiogenic activity of torilin, a sesquiterpene compound isolated from Torilis japonica",
abstract = "Torilin is a sesquiterpene compound purified from fruits of Torilis japonica (Umbelliferae). In this study, we demonstrated the anti-angiogenic activity of torilin using in vivo and in vitro assay systems. Torilin decreased both neovascularization of chick embryos in the chorioallantoic membrane assay and basic fibroblast growth factor-induced vessel formation in the mouse Matrigel plug assay. Torilin also reduced the proliferation and tube formation of human umbilical vein endothelial cells. In addition, the concentrated conditioned media obtained from torilin-treated HepG2 human hepatoblastoma cells blocked the angiogenic activation of torilin-untreated concentrated conditioned media, indicating that torilin may have an inhibitory effect on tumor-induced angiogenesis. To determine what molecules were involved in the anti-angiogenic activity, we examined the expression of hypoxia-inducible angiogenic factors in torilin-treated HepG2 cells. Torilin significantly down-regulated the expression of hypoxia-inducible vascular endothelial growth factor and insulin-like growth factor-11. Taken together, our data suggest that torilin may be a strong angiogenic inhibitor with the ability to decrease tube formation of vascular endothelial cells and to reduce expression of angiogenic factors of tumor cells. (C) 2000 Wiley-Liss, Inc.",
author = "Kim, {Myoung Sook} and Lee, {You Mie} and Moon, {Eun Joung} and Kim, {Se Eun} and Lee, {Jung Joon} and Kim, {Kyu Won}",
year = "2000",
month = "7",
day = "15",
doi = "10.1002/1097-0215(20000715)87:2<269::AID-IJC19>3.0.CO;2-W",
language = "English (US)",
volume = "87",
pages = "269--275",
journal = "International Journal of Cancer",
issn = "0020-7136",
publisher = "Wiley-Liss Inc.",
number = "2",

}

TY - JOUR

T1 - Anti-angiogenic activity of torilin, a sesquiterpene compound isolated from Torilis japonica

AU - Kim, Myoung Sook

AU - Lee, You Mie

AU - Moon, Eun Joung

AU - Kim, Se Eun

AU - Lee, Jung Joon

AU - Kim, Kyu Won

PY - 2000/7/15

Y1 - 2000/7/15

N2 - Torilin is a sesquiterpene compound purified from fruits of Torilis japonica (Umbelliferae). In this study, we demonstrated the anti-angiogenic activity of torilin using in vivo and in vitro assay systems. Torilin decreased both neovascularization of chick embryos in the chorioallantoic membrane assay and basic fibroblast growth factor-induced vessel formation in the mouse Matrigel plug assay. Torilin also reduced the proliferation and tube formation of human umbilical vein endothelial cells. In addition, the concentrated conditioned media obtained from torilin-treated HepG2 human hepatoblastoma cells blocked the angiogenic activation of torilin-untreated concentrated conditioned media, indicating that torilin may have an inhibitory effect on tumor-induced angiogenesis. To determine what molecules were involved in the anti-angiogenic activity, we examined the expression of hypoxia-inducible angiogenic factors in torilin-treated HepG2 cells. Torilin significantly down-regulated the expression of hypoxia-inducible vascular endothelial growth factor and insulin-like growth factor-11. Taken together, our data suggest that torilin may be a strong angiogenic inhibitor with the ability to decrease tube formation of vascular endothelial cells and to reduce expression of angiogenic factors of tumor cells. (C) 2000 Wiley-Liss, Inc.

AB - Torilin is a sesquiterpene compound purified from fruits of Torilis japonica (Umbelliferae). In this study, we demonstrated the anti-angiogenic activity of torilin using in vivo and in vitro assay systems. Torilin decreased both neovascularization of chick embryos in the chorioallantoic membrane assay and basic fibroblast growth factor-induced vessel formation in the mouse Matrigel plug assay. Torilin also reduced the proliferation and tube formation of human umbilical vein endothelial cells. In addition, the concentrated conditioned media obtained from torilin-treated HepG2 human hepatoblastoma cells blocked the angiogenic activation of torilin-untreated concentrated conditioned media, indicating that torilin may have an inhibitory effect on tumor-induced angiogenesis. To determine what molecules were involved in the anti-angiogenic activity, we examined the expression of hypoxia-inducible angiogenic factors in torilin-treated HepG2 cells. Torilin significantly down-regulated the expression of hypoxia-inducible vascular endothelial growth factor and insulin-like growth factor-11. Taken together, our data suggest that torilin may be a strong angiogenic inhibitor with the ability to decrease tube formation of vascular endothelial cells and to reduce expression of angiogenic factors of tumor cells. (C) 2000 Wiley-Liss, Inc.

UR - http://www.scopus.com/inward/record.url?scp=0010115240&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0010115240&partnerID=8YFLogxK

U2 - 10.1002/1097-0215(20000715)87:2<269::AID-IJC19>3.0.CO;2-W

DO - 10.1002/1097-0215(20000715)87:2<269::AID-IJC19>3.0.CO;2-W

M3 - Article

VL - 87

SP - 269

EP - 275

JO - International Journal of Cancer

JF - International Journal of Cancer

SN - 0020-7136

IS - 2

ER -