Anterior gradient-2 is overexpressed by fibrolamellar carcinomas

Perumal Vivekanandan, Shien T L Micchelli, Michael Torbenson

Research output: Contribution to journalArticle

Abstract

Anterior gradient-2 expression is critical in normal embryonic development. Aberrant expression of anterior gradient-2 in adult tissues has been linked to breast, prostate, esophageal, and pancreatic carcinoma. To define the role of anterior gradient-2 in primary hepatocellular neoplasms, we used tissue microarrays and examined protein expression in typical hepatocellular carcinomas (n = 44), fibrolamellar carcinomas (n = 12), and hepatic adenomas (n = 9). In nonneoplastic liver tissues, anterior gradient-2 was expressed in the septal-sized bile ducts and weakly in zone 3 hepatocytes in 11 (18%) of 61 cases. In tumors, anterior gradient-2 was overexpressed by only 1 (2%) of 44 hepatocellular carcinomas. In contrast, 6 (75%) of 8 fibrolamellar and 3 (75%) of 4 metastatic fibrolamellar carcinomas were positive. All 9 hepatic adenomas were negative. Further analysis of mRNA in fibrolamellar carcinomas identified 2 novel splice variants, but expression levels were very low. Sequencing of the anterior gradient-2 gene in fibrolamellar carcinomas identified several polymorphisms (refSNP Ids: rs6842, rs8071, rs1051905) but no mutations. In conclusion, anterior gradient-2 is overexpressed in the majority of fibrolamellar carcinomas but is only rarely overexpressed in hepatocellular carcinomas.

Original languageEnglish (US)
Pages (from-to)293-299
Number of pages7
JournalHuman Pathology
Volume40
Issue number3
DOIs
StatePublished - Mar 2009

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Carcinoma
Hepatocellular Carcinoma
Adenoma
Liver
Protein Array Analysis
Bile Ducts
Embryonic Development
Prostate
Hepatocytes
Neoplasms
Breast
Messenger RNA
Mutation
Genes

Keywords

  • Anterior gradient-2
  • Fibrolamellar carcinoma
  • Hepatic adenoma
  • Hepatocellular carcinoma

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Vivekanandan, P., Micchelli, S. T. L., & Torbenson, M. (2009). Anterior gradient-2 is overexpressed by fibrolamellar carcinomas. Human Pathology, 40(3), 293-299. https://doi.org/10.1016/j.humpath.2008.08.003

Anterior gradient-2 is overexpressed by fibrolamellar carcinomas. / Vivekanandan, Perumal; Micchelli, Shien T L; Torbenson, Michael.

In: Human Pathology, Vol. 40, No. 3, 03.2009, p. 293-299.

Research output: Contribution to journalArticle

Vivekanandan, P, Micchelli, STL & Torbenson, M 2009, 'Anterior gradient-2 is overexpressed by fibrolamellar carcinomas', Human Pathology, vol. 40, no. 3, pp. 293-299. https://doi.org/10.1016/j.humpath.2008.08.003
Vivekanandan, Perumal ; Micchelli, Shien T L ; Torbenson, Michael. / Anterior gradient-2 is overexpressed by fibrolamellar carcinomas. In: Human Pathology. 2009 ; Vol. 40, No. 3. pp. 293-299.
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