TY - JOUR
T1 - Anopheles stephensi salivary glands bear receptors for region I of the circumsporozoite protein of Plasmodium falciparum
AU - Sidjanski, Sacha P.
AU - Vanderberg, Jerome P.
AU - Sinnis, Photini
N1 - Funding Information:
The authors would like to thank Drs Victor Nussenzweig and Kenneth Vernick for helpful discussions and review of the manuscript, Jean Nonon for rearing the mosquitoes and Pedro Clavijo for peptide synthesis. We thank Drs David Kaslow and Bela Takacs for their generous gifts of recombinant Pfs25 and CS respectively. SPS was supported by the Fondation Medic pour la Recherche Medicale, Switzerland and by the Rotary Foundation. PS was supported by NIH grant no. K11AI0117501.
PY - 1997/12/1
Y1 - 1997/12/1
N2 - In the mosquito, Plasmodium sporozoites rupture from oocysts found on the midgut wall, circulate in the hemolymph and invade salivary glands where they wait to be injected into a vertebrate host during a bloodmeal. The mechanisms by which sporozoites specifically attach to and invade salivary glands are not known but evidence suggests that it is a receptor-mediated process. Here we show that the major surface protein of sporozoites, the circumsporozoite protein (CS), binds preferentially to salivary glands when compared to other organs exposed to the circulating hemolymph. In addition, we show that a peptide encompassing region I, a highly conserved sequence found in all rodent and primate Plasmodium CS proteins, inhibits binding of CS to mosquito salivary glands.
AB - In the mosquito, Plasmodium sporozoites rupture from oocysts found on the midgut wall, circulate in the hemolymph and invade salivary glands where they wait to be injected into a vertebrate host during a bloodmeal. The mechanisms by which sporozoites specifically attach to and invade salivary glands are not known but evidence suggests that it is a receptor-mediated process. Here we show that the major surface protein of sporozoites, the circumsporozoite protein (CS), binds preferentially to salivary glands when compared to other organs exposed to the circulating hemolymph. In addition, we show that a peptide encompassing region I, a highly conserved sequence found in all rodent and primate Plasmodium CS proteins, inhibits binding of CS to mosquito salivary glands.
KW - Circumsporozoite protein
KW - Malaria
KW - Mosquito
KW - Plasmodium
KW - Salivary glands
KW - Sporozoite
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U2 - 10.1016/S0166-6851(97)00124-2
DO - 10.1016/S0166-6851(97)00124-2
M3 - Article
C2 - 9497030
AN - SCOPUS:0031440944
SN - 0166-6851
VL - 90
SP - 33
EP - 41
JO - Molecular and Biochemical Parasitology
JF - Molecular and Biochemical Parasitology
IS - 1
ER -