Anopheles gambiae lacking AgTRIO inefficiently transmits Plasmodium berghei to mice

Yu Min Chuang, Marianna Freudzon, Jing Yang, Yuemei Dong, George Dimopoulos, Erol Fikrig

Research output: Contribution to journalArticle

Abstract

Antibodies to AgTRIO, a mosquito salivary protein, partially reduce the initial Plasmodium burden in mice. We therefore silenced AgTRIO in mosquitoes and determined the relative contribution of AgTRIO to the ability of Anopheles gambiae to transmit Plasmodium berghei to mice. RNA interference-mediated silencing of AgTRIO in A. gambiae resulted in a 60% reduction in AgTRIO expression. The decrease in AgTRIO expression did not alter the burden of Plasmodium sporozoites in mosquito salivary glands. When experimentally injected into mice, sporozoites from AgTRIO-silenced mosquitoes colonized the liver less effectively than sporozoites from control mosquitoes. Silencing of AgTRIO did not decrease the infectivity of sporozoites in vitro or influence the expression of genes associated with Plasmodium cell adhesion or traversal activity. AgTRIO decreased the expression of proinflammation cytokines by splenocytes in vitro. Moreover, in vivo, AgTRIO decreased the expression of TNF-α when coinjected with sporozoites into the skin and there was more TNF-α expression at the bite site of AgTRIO knockdown mosquitoes than at the bite site of control mosquitoes. AgTRIO therefore influences the local environment in the vertebrate host, which facilitates Plasmodium sporozoite infection in mice.

Original languageEnglish (US)
Article numbere00326-19
JournalInfection and Immunity
Volume87
Issue number9
DOIs
StatePublished - Sep 1 2019

Fingerprint

Anopheles gambiae
Plasmodium berghei
Sporozoites
Culicidae
Plasmodium
Mosquito Control
Bites and Stings
Salivary Proteins and Peptides
RNA Interference
Salivary Glands
Cell Adhesion
Malaria
Vertebrates
Cytokines
Gene Expression
Skin
Antibodies
Liver

Keywords

  • Anopheles gambiae
  • Inflammation
  • Malaria
  • Plasmodium
  • Saliva
  • Sporozoite

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

Cite this

Anopheles gambiae lacking AgTRIO inefficiently transmits Plasmodium berghei to mice. / Chuang, Yu Min; Freudzon, Marianna; Yang, Jing; Dong, Yuemei; Dimopoulos, George; Fikrig, Erol.

In: Infection and Immunity, Vol. 87, No. 9, e00326-19, 01.09.2019.

Research output: Contribution to journalArticle

Chuang, Yu Min ; Freudzon, Marianna ; Yang, Jing ; Dong, Yuemei ; Dimopoulos, George ; Fikrig, Erol. / Anopheles gambiae lacking AgTRIO inefficiently transmits Plasmodium berghei to mice. In: Infection and Immunity. 2019 ; Vol. 87, No. 9.
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