Anopheles gambiae Croquemort SCRBQ2, expression profile in the mosquito and its potential interaction with the malaria parasite Plasmodium berghei

Mónica González-Lázaro, Rhoel R. Dinglasan, Fidel de la Cruz Hernández-Hernández, Mario Henry Rodríguez, Martin Laclaustra, Marcelo Jacobs-Lorena, Leopoldo Flores-Romo

Research output: Contribution to journalArticle

Abstract

The scavenger receptor family comprises transmembrane proteins involved in the recognition of polyanionic ligands. Several studies have established that members of this family are involved both in immunity and in developmental processes. In Drosophila melanogaster, one of the best characterized scavenger receptors is Croquemort, which participates in the recognition of apoptotic cells in the embryo. Although comparative genomic studies have revealed the presence of four orthologs of this receptor in the malaria vector Anopheles gambiae, little is known about their function. We have investigated the expression pattern of the four Croquemort orthologs during the mosquito life cycle. Croquemort transcripts SCRBQ2 and SCRBQ4 are expressed at all the developmental stages, while expression of Croquemort transcripts SCRBQ1 and SCRBQ3 is more restricted. We have also investigated the expression of the four Croquemort orthologs in the different organs of the adult female. Croquemort transcript SCRBQ2 is highly expressed in the A. gambiae female midgut. SCRBQ2 midgut gene expression was up-regulated after a non-infected or a Plasmodium berghei-infected blood meal, compared to its expression in midguts of sugar-fed females. Interestingly, knockdown of SCRBQ2 expression by dsRNA injection resulted in a 62.5% inhibition of oocyst formation, suggesting that SCRBQ2 plays a role in Plasmodium-mosquito interactions.

Original languageEnglish (US)
Pages (from-to)395-402
Number of pages8
JournalInsect Biochemistry and Molecular Biology
Volume39
Issue number5-6
DOIs
StatePublished - May 2009

Fingerprint

Plasmodium berghei
Anopheles gambiae
Scavenger Receptors
Culicidae
midgut
malaria
Malaria
Parasites
parasites
receptors
Gene expression
Sugars
Life cycle
transmembrane proteins
Blood
Plasmodium
Oocysts
blood meal
Cells
double-stranded RNA

Keywords

  • Anopheles
  • CD36
  • Croquemort
  • RNAi
  • Scavenger receptor

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Insect Science

Cite this

Anopheles gambiae Croquemort SCRBQ2, expression profile in the mosquito and its potential interaction with the malaria parasite Plasmodium berghei. / González-Lázaro, Mónica; Dinglasan, Rhoel R.; Hernández-Hernández, Fidel de la Cruz; Rodríguez, Mario Henry; Laclaustra, Martin; Jacobs-Lorena, Marcelo; Flores-Romo, Leopoldo.

In: Insect Biochemistry and Molecular Biology, Vol. 39, No. 5-6, 05.2009, p. 395-402.

Research output: Contribution to journalArticle

González-Lázaro, Mónica ; Dinglasan, Rhoel R. ; Hernández-Hernández, Fidel de la Cruz ; Rodríguez, Mario Henry ; Laclaustra, Martin ; Jacobs-Lorena, Marcelo ; Flores-Romo, Leopoldo. / Anopheles gambiae Croquemort SCRBQ2, expression profile in the mosquito and its potential interaction with the malaria parasite Plasmodium berghei. In: Insect Biochemistry and Molecular Biology. 2009 ; Vol. 39, No. 5-6. pp. 395-402.
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