Liberación de endotelina-1 por angiotensina II en miocitos cardíacos aislados

Translated title of the contribution: Angiotensin II-induced endothelin-1 release in cardiac myocytes

María Celeste Villa-Abrille, Horacio E. Cingolani, Carolina D. Garciarena, Irene L. Ennis, Ernesto A. Aiello

Research output: Contribution to journalArticle

Abstract

Many of the effects thought to be due to angiotensin II (Ang II) are due to the release/formation of endothelin (ET). We tested whether Ang II elicits its positive inotropic effect (PIE) by the action of endogenous ET-1 and the role played by the reactive oxygen species (ROS) in this mechanism. Experiments were performed in cat isolated ventricular myocytes in which sarcomere shortening (SS) was measured to asses contractility after pharmacological interventions and the effect of Ang II on inotropism were analyzed. Ang II 1 nM increased SS by 31.8±3.8% (p<0.05). This PIE was cancelled by AT1 receptor blockade, by ET-1 receptors blockade, by Na+/H+ exchanger (NHE) inhibition, by reverse mode Na+/Ca2+. exchanger (NCX) blockade or by ROS scavenging. Ang II 100 nM increases SS by 70.5±7.6% (p<0.05). This PIE was completely abolished by AT1 receptors blockade and were partially bocked by ET-1 receptors blockade, by NHE inhibition, by reverse mode NCX blockade or by ROS scavenging. Ang II increased preproET-1 mRNA, effect that was blunted by AT1 receptors blockade. We conclude that Ang II induces (through its AT1 receptor) release/formation of ET-1, which acting in autocrine fashion on ET receptors of the isolated myocytes increases inotropism through NHE stimulation and NCX reverse mode activation. The participation of ROS is involved is this chain of events.

Translated title of the contributionAngiotensin II-induced endothelin-1 release in cardiac myocytes
Original languageSpanish
Pages (from-to)229-236
Number of pages8
JournalMedicina
Volume66
Issue number3
StatePublished - Jul 28 2006

Keywords

  • Angiotensin II
  • Endothelin
  • Na/Ca exchanger
  • Na/H exchanger
  • Reactive oxygen species

ASJC Scopus subject areas

  • Medicine(all)

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  • Cite this

    Villa-Abrille, M. C., Cingolani, H. E., Garciarena, C. D., Ennis, I. L., & Aiello, E. A. (2006). Liberación de endotelina-1 por angiotensina II en miocitos cardíacos aislados. Medicina, 66(3), 229-236.