Angiotensin II-acetylcholine noncovalent complexes analyzed with MALDI-ion mobility-TOF MS

Amina S. Woods, Katrin Fuhrer, Marc Gonin, Tom Egan, Michael Ugarov, Kent J. Gillig, J. Albert Schultz

Research output: Contribution to journalArticlepeer-review

Abstract

Matrix-assisted laser desorption ionization-ion mobility- orthogonal time-of-flight mass spectrometry (MALDI-IM-oTOF MS) is a new technique that allows laser desorbed ions to be preseparated on the basis of their shape prior to mass analysis. Using this instrument, we tested the postulate that addition of a quaternary ammonium compound such as acetylcholine to the model phosphorylated peptide angiotensin II would enhance its detection by MALDI in two ways. First of all, the acetylcholine-peptide complex could ionize more efficiently than the bare phosphopeptide. Furthermore, the ion mobility could separate the complex ion on the basis of its charge/volume from isobaric interferences, which would otherwise limit detection sensitivity.

Original languageEnglish (US)
Pages (from-to)1-8
Number of pages8
JournalJournal of Biomolecular Techniques
Volume14
Issue number1
StatePublished - Mar 2003
Externally publishedYes

Keywords

  • Acetylcholine
  • Angiotensin
  • Ion mobility
  • Phosphorylated pep-tides

ASJC Scopus subject areas

  • Molecular Biology

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