Angiotensin-converting enzyme localized in the rat pituitary and adrenal glands by [3h]captopril autoradiography

Stephen M. Strittmatter, Errol B. De Souza, David R. Lynch, Solomon H. Snyder

Research output: Contribution to journalArticle

Abstract

We have localized angiotensin-converting enzyme (EC 3.14.5.1) in the rat pituitary and adrenal glands by [3H]captopril autoradiography. The maximal numbers of [3H] captopril-binding sites are: Posterior pituitary, 4500 fmol/mg protein; anterior pituitary, 1950 fmol/mg; intermediate pituitary, <100 fmol/mg; adrenal medulla, 480 fmol/mg; and adrenal cortex, <25 fmol/mg. The distribution within the posterior pituitary and adrenal medulla is homogeneous, whereas that in the anterior pituitary is patchy. Subcellular fractionation of the bovine adrenal medulla reveals enrichment of angiotension-converting enzyme in plasma membrane fractions, but not in chromaffin granules. [3H]Captopril autoradiography in the rat pituitary gland is unaltered by dehydration, adrenalectomy, or reserpine treatment and in Brattleboro rats. [3H]Captopril binding in the adrenal medulla is increased by 75% 3 weeks after hypophysectomy and is elevated by 80% after reserpine treatment. The change after hypophysectomy is not reversed by dexamethasone treatment.

Original languageEnglish (US)
Pages (from-to)1690-1699
Number of pages10
JournalEndocrinology
Volume118
Issue number4
DOIs
StatePublished - Jan 1 1986

ASJC Scopus subject areas

  • Endocrinology

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