Angiotensin-converting enzyme localized in the rat pituitary and adrenal glands by [3H]captopril autoradiography

S. M. Strittmatter, E. B. De Souza, D. R. Lynch, Solomon H Snyder

Research output: Contribution to journalArticle

Abstract

We have localized angiotensin-converting enzyme (EC 3.14.5.1) in the rat pituitary and adrenal glands by [3H]captopril autoradiography. The maximal numbers of [3H] captopril-binding sites are: posterior pituitary, 4500 fmol/mg protein; anterior pituitary, 1950 fmol/mg; intermediate pituitary, <100 fmol/mg; adrenal medulla, 480 fmol/mg; and adrenal cortex, <25 fmol/mg. The distribution within the posterior pituitary and adrenal medulla is homogeneous, whereas that in the anterior pituitary is patchy. Subcellular fractionation of the bovine adrenal medulla reveals enrichment of angiotensin-converting enzyme in plasma membrane fractions, but not in chromaffin granules. [3H]Captopril autoradiography in the rat pituitary gland is unaltered by dehydration, adrenalectomy, or reserpine treatment and in Brattleboro rats. [3H]Captopril binding in the adrenal medulla is increased by 75% 3 weeks after hypophysectomy and is elevated by 80% after reserpine treatment. The change after hypophysectomy is not reversed by dexamethasone treatment.

Original languageEnglish (US)
Pages (from-to)1690-1699
Number of pages10
JournalEndocrinology
Volume118
Issue number4
StatePublished - 1986

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Adrenal Medulla
Captopril
Peptidyl-Dipeptidase A
Pituitary Gland
Adrenal Glands
Autoradiography
Hypophysectomy
Reserpine
Brattleboro Rats
Chromaffin Granules
Adrenal Cortex
Adrenalectomy
Dehydration
Dexamethasone
Binding Sites
Cell Membrane
Proteins

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Angiotensin-converting enzyme localized in the rat pituitary and adrenal glands by [3H]captopril autoradiography. / Strittmatter, S. M.; De Souza, E. B.; Lynch, D. R.; Snyder, Solomon H.

In: Endocrinology, Vol. 118, No. 4, 1986, p. 1690-1699.

Research output: Contribution to journalArticle

Strittmatter, S. M. ; De Souza, E. B. ; Lynch, D. R. ; Snyder, Solomon H. / Angiotensin-converting enzyme localized in the rat pituitary and adrenal glands by [3H]captopril autoradiography. In: Endocrinology. 1986 ; Vol. 118, No. 4. pp. 1690-1699.
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