TY - JOUR
T1 - Angiotensin converting enzyme immunohistochemistry in rat brain and pituitary gland
T2 - Correlation of isozyme type with cellular localization
AU - Strittmatter, S. M.
AU - Snyder, S. H.
N1 - Funding Information:
These studies suggest distinct functions for different isozymic fot&s of ACE. Similar variations in ACE isozymes and apparent physiologic roles occur in the male reproductive system39a nd the adrenal medulla” (Table 1). Conceivably, ACE inhibitors with isozvmic selectivitv mav exert selective I < pharmacologic aciions, including behavioral effects.47 Acknowledgements-We thank Dr M. M. S. Lo for nro-duction of-the monoclonala ntibodyand for helpful-discussions,D r E. B. De Souzaf or a gift of pituitarys ections from stalk transecteda nimals,D r K. B. Braas for advice regardingi mmunohistochemicaplr oceduresD, . R. Lynch for criticalr eviewo f the manuscrinta. nd Dawn C. Dodson for secretariaal ssistanceT.h is wo&is supportedb y N.I.H. Training Grant GM-07309t o S.M.S. USPHS grant DA-00266a nd ResearchS cientistA ward DA-00074 to S.H.S.
Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1987/5
Y1 - 1987/5
N2 - We have localized angiotensin converting enzyme in rat brain and pituitary gland immunohistochemically with an anti-rat lung angiotensin converting enzyme monoclonal antibody. The distribution of immunoreactive angiotensin converting enzyme is identical with that of binding sites for the angiotensin converting enzyme inhibitor, [3H]captopril. Most intense staining is in the choroid plexus and subfornical organ, with intermediate values in the caudate-putamen, globus pallidus, entopeduncular nucleus, pars reticulata of the substantia nigra, posterior pituitary and anterior pituitary. Lower levels are observed in the supraoptic and paraventricular nuclei of the hypothalamus. Within the basal ganglia angiotensin converting enzyme immunoreactivity is distributed throughout the neuropil; no cell bodies are stained, even after colchicine treatment. The punctate pattern of immunoreactivity in the anterior pituitary corresponds to the distribution of endothelial cells. The posterior pituitary is stained diffusely. Angiotensin converting enzyme is increased by 45% in the posterior lobe after pituitary stalk section, demonstrating that this diffuse staining is associated with pituicytes. Antibody specificity was demonstrated by the immunoaffinity purification of angiotensin converting enzyme to homogeneity from crude tissue extracts using anti-angiotensin converting enzyme antibody and protein A-sepharose. The apparent molecular weight by sodium dodecyl sulfate polyacrylamide gel electrophoresis of lung, choroid plexus and anterior pituitary angiotensin converting enzyme is 175,000. In the substantia nigra and caudate putamen, where angiotensin converting enzyme is localized to neuronal as opposed to epithelial cells, the molecular weight is 165,000. The pituicyte angiotensin converting enzyme of the posterior pituitary is 170,000 dallons.
AB - We have localized angiotensin converting enzyme in rat brain and pituitary gland immunohistochemically with an anti-rat lung angiotensin converting enzyme monoclonal antibody. The distribution of immunoreactive angiotensin converting enzyme is identical with that of binding sites for the angiotensin converting enzyme inhibitor, [3H]captopril. Most intense staining is in the choroid plexus and subfornical organ, with intermediate values in the caudate-putamen, globus pallidus, entopeduncular nucleus, pars reticulata of the substantia nigra, posterior pituitary and anterior pituitary. Lower levels are observed in the supraoptic and paraventricular nuclei of the hypothalamus. Within the basal ganglia angiotensin converting enzyme immunoreactivity is distributed throughout the neuropil; no cell bodies are stained, even after colchicine treatment. The punctate pattern of immunoreactivity in the anterior pituitary corresponds to the distribution of endothelial cells. The posterior pituitary is stained diffusely. Angiotensin converting enzyme is increased by 45% in the posterior lobe after pituitary stalk section, demonstrating that this diffuse staining is associated with pituicytes. Antibody specificity was demonstrated by the immunoaffinity purification of angiotensin converting enzyme to homogeneity from crude tissue extracts using anti-angiotensin converting enzyme antibody and protein A-sepharose. The apparent molecular weight by sodium dodecyl sulfate polyacrylamide gel electrophoresis of lung, choroid plexus and anterior pituitary angiotensin converting enzyme is 175,000. In the substantia nigra and caudate putamen, where angiotensin converting enzyme is localized to neuronal as opposed to epithelial cells, the molecular weight is 165,000. The pituicyte angiotensin converting enzyme of the posterior pituitary is 170,000 dallons.
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U2 - 10.1016/0306-4522(87)90131-X
DO - 10.1016/0306-4522(87)90131-X
M3 - Article
C2 - 3039401
AN - SCOPUS:0023197247
SN - 0306-4522
VL - 21
SP - 407
EP - 420
JO - Neuroscience
JF - Neuroscience
IS - 2
ER -