Angiopoietin-2 Enhances Retinal Vessel Sensitivity to Vascular Endothelial Growth Factor

Yuji Oshima, Tye Deering, Sachiko Oshima, Hiroyuki Nambu, P. Seshidhar Reddy, Michael Kaleko, Sheila Connelly, Sean F. Hackett, Peter A Campochiaro

Research output: Contribution to journalArticle

Abstract

Increased expression of vascular endothelial growth factor (VEGF) in the retina starting after postnatal day (P)7 results in neovascularization originating from deep retinal capillaries, but not those in the superficial capillary bed. Doxycycline was administered starting P0 to double transgenic mice with inducible expression of VEGF in the retina. These mice showed proliferation and dilation of superficial retinal capillaries, indicating that at this stage of development, the superficial capillaries are sensitive to the effects of VEGF. Angiopoietin-2 (Ang2) is expressed along the surface of the retina for several days after birth, but by P7 and later, Ang2 is only expressed in the region of the deep capillary bed. In mice with ubiquitous doxycycline-inducible expression of Ang2, in the absence of doxycycline, intravitreous injection of a gutless adenoviral vector expressing VEGF (AGV.VEGF) resulted in neovascularization of the cornea and iris, but no retinal neovascularization. After treatment with doxycycline to induce Ang2 expression, intravitreous injection of AGV.VEGF caused retinal neovascularization in addition to corneal and iris neovascularization. The retinal neovascularization originated from both the superficial and deep capillary beds. These data suggest that Ang2 promotes sensitivity to the angiogenic effects of VEGF in retinal vessels.

Original languageEnglish (US)
Pages (from-to)412-417
Number of pages6
JournalJournal of Cellular Physiology
Volume199
Issue number3
DOIs
StatePublished - Jun 2004

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Angiopoietin-2
Retinal Vessels
Vascular Endothelial Growth Factor A
Doxycycline
Retinal Neovascularization
Retina
Iris
Corneal Neovascularization
Injections
Cornea
Transgenic Mice
Dilatation
Parturition

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Cite this

Angiopoietin-2 Enhances Retinal Vessel Sensitivity to Vascular Endothelial Growth Factor. / Oshima, Yuji; Deering, Tye; Oshima, Sachiko; Nambu, Hiroyuki; Reddy, P. Seshidhar; Kaleko, Michael; Connelly, Sheila; Hackett, Sean F.; Campochiaro, Peter A.

In: Journal of Cellular Physiology, Vol. 199, No. 3, 06.2004, p. 412-417.

Research output: Contribution to journalArticle

Oshima, Y, Deering, T, Oshima, S, Nambu, H, Reddy, PS, Kaleko, M, Connelly, S, Hackett, SF & Campochiaro, PA 2004, 'Angiopoietin-2 Enhances Retinal Vessel Sensitivity to Vascular Endothelial Growth Factor', Journal of Cellular Physiology, vol. 199, no. 3, pp. 412-417. https://doi.org/10.1002/jcp.10442
Oshima, Yuji ; Deering, Tye ; Oshima, Sachiko ; Nambu, Hiroyuki ; Reddy, P. Seshidhar ; Kaleko, Michael ; Connelly, Sheila ; Hackett, Sean F. ; Campochiaro, Peter A. / Angiopoietin-2 Enhances Retinal Vessel Sensitivity to Vascular Endothelial Growth Factor. In: Journal of Cellular Physiology. 2004 ; Vol. 199, No. 3. pp. 412-417.
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