Angiogenesis is stimulated by a tumor‐derived endothelial cell growth factor

Yuen Shing, Judah Folkman, Christian Haudenschild, Dennis Lund, Rosa Crum, Michael Klagsbrun

Research output: Contribution to journalArticlepeer-review

Abstract

A growth factor mitogenic for BALB/C 3T3 cells and capillary endothelial cells was isolated from a rat chondrosarcoma and purified to homogeneity. Purification was accomplished by a combination of BioRex 70 cation exchange chromatography and heparin affinity chromatography. The pure chondrosarcoma‐derived growth factor (ChDGF) had a molecular weight of about 18.000. The angiogenesis activity of pure ChDGF was tested by measuring its ability to vascularize the chorioallantoic membrane (CAM) and yolk sac membrane of the developing chick. The ability of ChDGF to induce the growth of limbal vessels in the rat cornea was also measured. To quantitate the angiogenesis response, a unit system based on the growth factor activity of ChDGF for 3T3 cells was adopted. ChDGF was found to have a specific activity of about 5 units/ng when applied to 3T3 cells. About 300–600 units of ChDGF in the two types of developing chick membrane and 30–50 units of ChDGF in the rat cornea were found to stimulate noninflammatory angiogenesis.

Original languageEnglish (US)
Pages (from-to)275-287
Number of pages13
JournalJournal of cellular biochemistry
Volume29
Issue number4
DOIs
StatePublished - 1985
Externally publishedYes

Keywords

  • angiogenesis
  • chondrosarcoma
  • endothelial cell
  • growth factor
  • heparin

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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